%0 Journal Article
%T Isolation,identification and enzyme profiling of an alkaliphilic protease producing bacterium<br>一株产蛋白酶嗜碱菌株的分离、鉴定及酶学特性
%A Jianguo Hao
%A Yanfen Xue
%A Yanhe Ma
%A <br>郝建国
%A 薛燕芬
%A 马延和
%J 微生物学报
%D 2010
%I 
%X Objective] To isolate and characterize alkaliphilic bacteria with protease activity. Methods] Protease-producing alkaliphiles were isolated by skim milk agar method. The morphological,biochemical and physiological characteristics,16S rRNA gene sequence and DNA-DNA hybridization were determined to identify the taxonomic position of strain ZL223. Meanwhile,the enzyme characterization of the protease including optimal temperature and pH range,stability and oxidation resistance were tested by using casein hyd...
%K Alkaliphile
%K Bacillus pseudofirmus
%K 16S rRNA gene
%K DNA-DNA hybridization<br>关键字：嗜碱微生物
%K 假强芽孢杆菌
%K 16S
%K rRNA
%K 基因
%K DNA–DNA杂交
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=4E0982F8027A63604452C61A71D6CDB2&yid=140ECF96957D60B2&vid=771152D1ADC1C0EB&iid=CA4FD0336C81A37A&sid=318E4CC20AED4940&eid=6AC2A205FBB0EF23&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=0