%0 Journal Article
%T In vitro function of outer membrane protease T of Escherichia coli K1 pathogenic strain<br>大肠杆菌K1致病株外膜蛋白T体外功能
%A Changye Hui
%A Yan Guo
%A Shuchi Wu
%A Liang Peng
%A Hong Cao
%A Shenghe Huang
%A <br>惠长野
%A 郭妍
%A 吴书驰
%A 彭亮
%A 曹虹
%A 黄胜和
%J 微生物学报
%D 2010
%I 
%X Plasminogen activation and antimicrobial peptide hydrolysis contribute to pathogens invasion and survival in vivo. Objective] To demonstrate the expression of outer membrane protease T in E. coli K1 pathogenic strain E44,its activity of plasminogen activator and protamine hydrolysis. Methods] After Benzamidine Sepharose Fast Flow and SOURCE 30Q chromatography,we got E44 outer membrane mixed fraction,and examined its activity of plasminogen activation with chromogenic substrate S-2251 method. An ompT delet...
%K Keywords: outer membrane protease T (OmpT)
%K plasminogen
%K activation
%K protamine
%K hydrolysis<br>关键词：外膜蛋白T
%K 纤溶酶原
%K 激活
%K 鱼精蛋白
%K 水解
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=AB557A54965601913AD948449A302293&yid=140ECF96957D60B2&vid=771152D1ADC1C0EB&iid=CA4FD0336C81A37A&sid=B6DA1AC076E37400&eid=8E6AB9C3EBAAE921&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=0