%0 Journal Article
%T Replication and conjugation of Streptomyces small plasmid pDYM4. 3k<br>链霉菌小质粒pDYM4.3k的复制与接合转移
%A Min Zhou
%A Yumei Dai
%A Li Zhong
%A Zhongjun Qin
%A <br>周敏
%A 代玉梅
%A 钟莉
%A 覃重军
%J 微生物学报
%D 2012
%I 
%X Objective] Streptomyces sp.X335,harboring a 4.3-kb plasmid pDYM4.3k,was isolated from Tibet sample.Cloning,sequencing,analyzing and functional investigations of pDYM4.3k.Methods] Prime walking to obtain plasmid sequence,BLAST to predict gene function,Southern hybridization to determine replication intermediates,and mating to test conjugal function of plasmid.Results] The complete nucleotide sequence of pDYM4.3k consisted of 4346 bp,encoding 3 genes of which one resembled Streptomyces major conjugative gene tra and other two were with unknown functions.Experiments demonstrated that a new gene of pDYM4.3k and its upstream c.300 bp were required for plasmid replication.Single-stranded pDYM4.3k as a replication intermediate was detected,indicating it rolling-cirle replication mode.pDYM4.3k could conjugal transfer among Streptomyces lividans.Conclusion] Streptomyces small plasmid pDYM4.3k was able to autonomous replication and conjugal transferring.
%K Streptomyces
%K plasmid
%K replication
%K conjugation<br>链霉菌
%K 质粒
%K 复制
%K 接合转移
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=8324B84C7395C656784FFE68F3042920&yid=99E9153A83D4CB11&vid=286FB2D22CF8D013&iid=DF92D298D3FF1E6E&sid=DE4E739E935BD9A7&eid=753F7A67351FADCC&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=0