%0 Journal Article
%T Identification and characteristics of a marine aerobic denitrifying bacterium<br>一株海洋好氧反硝化细菌的鉴定及其好氧反硝化特性
%A Xiyan Gao
%A Ying Liu
%A Haiyan Zheng
%A Ying Liu
%A Zhipei Liu
%A <br>高喜燕
%A 刘鹰
%A 郑海燕
%A 刘缨
%A 刘志培
%J 微生物学报
%D 2010
%I 
%X Abstract: Objective] An aerobic denitrifying bacterium, strain 2-8, was isolated from a biological aerated filter in a recirculating marine aquaculture system, phylogeny and characteristics of the strain was further studied. Methods] Sequence of the 16S rRNA gene was analyzed and the factors affect the denitrifying ability of strain 2-8 were investigated, including carbon source, C/N ratio, initial pH, NaCl concentration, temperature and shaker speed. Results] It was identified as Pseudomonas sp. 2-8 based on the analysis of its 16S rRNA gene sequence which showed highest similarity (99.9%) to Pseudomonas segetis FR1439T (AY770691). The results indicated that carbon source and C/N ratio exhibited significant influences on aerobic denitrifying capacity of strain 2-8. Strain 2-8 could grow a little on acetate, succinate and citrate as sole carbon source, and the removal rates of NO3--N at 140 mg/L were higher than 65% despite of the accumulation of NO2--N around 35 mg/L. It grew quite well on glucose as sole carbon source, however, the removal rate of nitrate was not so high as on other carbon source. The optimum C/N ratio was 15, as lower C/N ratios may lead to nitrite accumulation. The optimum temperature and pH for its aerobic denitrification were 30 oC and 7.5, respectively. Strain 2-8 could grow and exhibit aerobic denitrifying ability at a wide range of NaCl concentrations (0-30 g/L). The highest nitrogen removal appeared under the condition of 160 r/min shaking culture. Conclusion] When cultured in the conditions of NO3--N at 140 mg/L, sodium citrate as sole carbon source, C/N ratio at 15, pH 7.5, NaCl at 30g/L, 30 oC and 160 r/min of the shaker, strain 2-8 removed up to 92% of the nitrogen within 48 hours, and no nitrite accumulation.
%K Keywords: marine environments
%K nitrogen removal
%K biological denitrification
%K aerobic denitrifier<br>关键词：海洋环境
%K 氮素去除
%K 生物脱氮
%K 好氧反硝化菌
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=4E77D8DD9B873445D47FEFC4DC8CB404&yid=140ECF96957D60B2&vid=771152D1ADC1C0EB&iid=9CF7A0430CBB2DFD&sid=4E85BC78FC25985C&eid=D610BE6A5A0C75BF&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=0