%0 Journal Article
%T Effect of mutation of chemotaxis signal transduction gene cheA in Pseudomonas putida DLL-1 on its chemotaxis and methyl parathion biodegradation<br>趋化信号转导基因cheA突变对Pseudomonas putida DLL-1甲基对硫磷的趋化性及原位降解的影响
%A WEN Yang
%A JIANG Jian-dong
%A DENG Hai-hu
%A LAN Hong
%A LI Shun-peng
%A <br>文阳
%A 蒋建东
%A 邓海华
%A 蓝鸿
%A 李顺鹏
%J 微生物学报
%D 2007
%I 
%X Pseudomonas putida DLL-1 is a high effective methyl parathion (MP) degrading strain, which is also of chemotaxis to MP. cheA, responsible for coding histidine kinase, plays an important role in bacterial chemotaxis signal transduction. In order to study the effect of bacterial chemotaxis on in-situ biodegradation of pesticides, cheA in chromosome of P. putida DLL-1 was mutated by gene-targeting and successfully obtained a MP-chemotaxis mutant DAK. The mutant DAK shows the same growth ability as wild-type DLL-1 in LB medium. The degrading rate of 50 mg/kg MP in non-sterile and sterile soil of chemotaxis mutant DAK is about 20%-30% lower than that of wild-type bacterial DLL-1. Lose of chemotaxis in DLL-1 would decline its degradation ability of MP. It was demonstrated that chemotaxis plays an important role in the in-situ biodegradation of pesticides.
%K Chemotaxis
%K Pesticide biodegradation
%K Pseudomonas putida DLL-1
%K cheA: Methyl parathionv<br>趋化性
%K 农药降解
%K Pseudomonas
%K DLL-1
%K cheA基因
%K 甲基对硫磷
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=33A0CA6123942BF3&yid=A732AF04DDA03BB3&vid=F4B561950EE1D31A&iid=38B194292C032A66&sid=DCE57F652E4ADAFC&eid=DA4893B5F9885621&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=18