%0 Journal Article
%T Sequence of Escherichia coli O138 O-antigen Gene Cluster and Gne Identification by Bioinformatics<br>大肠杆菌O138 O-抗原基因簇的破译及Gne的生物信息学鉴定
%A Qing-Ke Kong
%A Jian-Song Cheng
%A Guang Zhao
%A Lei Wang
%A Hong-Jie Guo
%A <br>孔庆科
%A 程剑松
%A 赵广
%A 王磊
%A 郭宏杰
%J 微生物学报
%D 2004
%I 
%X E. coli O138 is one of the enterotoxigenic Escherichia coli, causing the postweaning diarrhea and edema disease of weaned pigs. The O-antigen gene cluster of E. coli O138 was sequenced and found to contain the genes rmlBDAC and gne, gna for the biosynthesis of nucleotide sugars dTDP-rhamnose and UDP-GalNAcA, respectively, genes encoding for O unit flippase(wzx), O-antigen polymerase(wzy) and 3 potential transferase genes. The possible biosynthesis pathway for rare UDP-GalNAcA was proposed. Two genes specific to E. coli O138 were identified. This work provides the basis for a sensitive test by PCR for the rapid detection of E. coli O138. Phylogenetic tree for Gne and GalE proteins was generated and comparisons were made among different strains, and results revealed that these proteins are similar in the second structure, and Gne of E. coli O138 was identified by bioinformatics.
%K Escherichia coli O138
%K O-antigen
%K Specific gene
%K Gne<br>大肠杆菌O138
%K O-抗原
%K 特异基因
%K UDP-GlcNAc
%K C4异构酶
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=6F2B8C4FD0249F73&yid=D0E58B75BFD8E51C&vid=1AE5323881A5ECDC&iid=B31275AF3241DB2D&sid=895BB10EB8F3BD49&eid=8D71AF42ACD39979&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=11