%0 Journal Article
%T Effect of exopolysaccharide from Bifidobacterium bifidum on cell of gastric cancer and hTERT<br>两歧双歧杆菌胞外多糖对胃癌细胞及hTERT的影响
%A Xu Chen
%A Hongrui Jiang
%A Yanmei Yang
%A Ning Liu
%A <br>陈旭
%A 江虹锐
%A 杨艳梅
%A 刘宁
%J 微生物学报
%D 2009
%I 
%X Objective] To study the inhibition of human gastric cancer cell BGC823 by exopolysaccharide (B. EPS) extracted from Bifidobacterium bifidum and the effect on the activity of telomerase rate-limiting factor hTERT. Methods] The B. EPS was divided into three groups by multiplication concentration level which effected on human gastric cancer cell BGC823 in vitro, the inhibition rate of gastric cancer cell BGC-823, the morphology changes of cells and the apoptosis in the initial stage of cell was observed respectively by MTT, inverted fluorescent microscope and the Annexin V-FITC/PI flow cytometry. In addition, the effect of B.EPS on cell telomerase rate-limiting factor hTERT mRNA was tested by RT-PCR; the changes in the concentration of calcium ions inside the cytoplasm were detected by spectrofluorometer. Results] B.EPS inhibited the growth of gastric cancer cell BGC-823 (P<0.05), the inhibition rate increased and the concentration of B.EPS showed dose-time response relation. The expression of cell telomerase rate-limiting factor hTERT mRNA decrease after effected by B.EPS (P<0.05), and showed dose-effect relation. The concentration of calcium ion in cytoplasm was higher than the control apparently (P<0.05). Conclusion] The apoptosis mechanism of human gastric cancer cell BGC823 induced by B.EPS may be relevant with the expression of hTERT mRNA and the concentration of calcium ions in cytoplasm.
%K Bifidobacterium
%K Exopolysaccharide
%K Gastric cancer
%K Apoptosis
%K hTERT<br>两歧双歧杆菌
%K 胞外多糖
%K 胃癌
%K 细胞凋亡
%K hTERT
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=938FC8393690B4BA16B58AC1B67EF126&yid=DE12191FBD62783C&vid=2A3781E88AB1776F&iid=CA4FD0336C81A37A&sid=7555FB9CC973F695&eid=B62E0EEFE746E568&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=0