%0 Journal Article
%T Cloning of a Homologous Gene of Magnaporthe grisea PMK1 Type MAPK from Ustilaginoidea virens and Functional Identification by Complement in Magnaporthe grisea Corresponding Mutant<br>稻曲病菌PMK1类同源基因克隆及在稻瘟病菌遗传互补中的功能验证
%A Zhen Zhang
%A Xinfa Du
%A Rongyao Chai
%A Jiaoyu Wang
%A Haiping Qiu
%A Xueqin Mao
%A Guochang Sun
%A <br>张震
%A 杜新法
%A 柴荣耀
%A 王教瑜
%A 邱海萍
%A 毛雪琴
%A 孙国昌
%J 微生物学报
%D 2008
%I 
%X 目的]克隆稻曲病菌PMK1类MAPK(Mitogen-activated protein kinase)同源基因.方法]根据丝状真菌MAPK蛋白保守性设计简并引物扩增稻曲病菌MAPK基因部分片段,进而利用TAIL-PCR进行染色体步移和RT-PCR获得UVMK1基因全长和cDNA全长.构建互补载体,交叉互补稻瘟病菌APMK1突变体菌株nn78进行功能验证,包括附着胞分化和致病性测定.结果]UVMK1基因全长1435 bp,包含3个内含子,编码355氨基酸的蛋白.UVMK1推导蛋白与丝状真菌Magnaporthe grisea PMK1,Fusarium oxysporum FMK1,Fusarium solani FSMAPK,Colletotrichumlagenarium CMK1,Botrytis cinerea BMK1,Claviceps purpurea CMPK1等编码蛋白高度同源.转化稻瘟病菌菌株nn78,获得5个转化子.其中选取的转化子恢复了稻瘟病菌正常的附着胞分化和对大麦叶片的致病能力.结论]本研究成功分离了首个稻曲病菌MAPK基因,而且UVMK1基因是稻瘟病菌PMK1的同源基因.
%K UVMK1
%K MAPK<br>稻曲病菌
%K 稻瘟病菌
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=C45321A173B520A96929EB2346EA60CC&yid=67289AFF6305E306&vid=B6DA1AC076E37400&iid=708DD6B15D2464E8&sid=B2A9EA001599F2ED&eid=9F9D3173F7EC760A&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=22