%0 Journal Article
%T Research on the classification of Streptomyces strain ZG0429 and purification of streptavidin<br>链霉菌ZG0429的分类鉴定与链霉亲和素的分离纯化研究
%A ZHANG Lei
%A LIU Chun-qin
%A GAO Zhi-hui
%A CAO Yu
%A BAI Gang
%A <br>张蕾
%A 刘春琴
%A 高智慧
%A 曹宇
%A 白钢
%J 微生物学报
%D 2007
%I 
%X Actinomycete strain ZG0429 with high production of streptavidin was isolated from soil samples. Based on the results of morphological, physiological, chemotaxonomic characteristics and 16S rRNA analysis, strain ZG0429 was placed within the genus Streptomyces and identified as the species Streptomyces lavendulae. The production reached 201.0mg/L under our fermentation conditions. With the effective system of ammonium_sulfate deposition and gel filtration chromatography, streptavidin was successfully purified from the culture supernatant with an overall recovery of 76.87% and purity of 97.03%. This study illustrates an effective and inexpensive method to produce the aimed protein with favorable yield, high purity and desirable activity, so it provides a valuable reference for the large_scale industrial production and isolation of streptavidin.
%K streptavidin
%K Streptomyces lavendulae
%K classification and characterization
%K isolation and purification<br>链霉亲和素
%K 淡紫灰链霉菌
%K 分类鉴定
%K 分离纯化
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=2DFAEBBFB3C13203&yid=A732AF04DDA03BB3&vid=F4B561950EE1D31A&iid=CA4FD0336C81A37A&sid=DF92D298D3FF1E6E&eid=F3090AE9B60B7ED1&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=15