%0 Journal Article
%T Simultaneous detection of enterovirus 71 and coxsackievirus A16 by multiplex real-time PCR with an internal amplification control<br>内标多重荧光RT-PCR同时检测肠道病毒71型和柯萨奇病毒A16型
%A Xinglong Xiao
%A Yaqing He
%A Yigang Yu
%A Hongyang
%A Huifang Li
%A Xiaoquan Yang
%A Jingwei Zhang
%A Guchen
%A Dongmei Liu
%A Xiaofeng Li
%A Hui Wu
%A <br>肖性龙
%A 何雅青
%A 余以刚
%A 杨洪
%A 李惠芳
%A 杨晓泉
%A 张经纬
%A 陈谷
%A 刘冬梅
%A 李小凤
%A 吴晖
%J 微生物学报
%D 2009
%I 
%X Objective] To rapidly identify EV71 and CA16 simultaneously. Methods] A multiplex real-time PCR with an internal control (IC) was developed. The specificity, sensitivity, reproducibility of the real time RT-PCR assay were estimated and more over 400 clinical samples were tested. Results] The results showed 100% specificity for the selected panel. The assay met the sensitivity of 50% tissue culture infective dose (TCID50) per milliliter samples for CA16 and 0.1 TCID50 for EV71. Analysis with 104-0.1TCID50/mL EV71 or CA16 samples demonstrated high reproducibility with a coefficient of variation (CV) of 0.9-2.0% for EV71 and 0.9-2.3% for CA16. More than 400 clinical samples were detected by real-time PCR, The results showed that the average positive ratio for EV71 and CA16 were 46.1% and 14.2%, higher than common assay (34.5% and 12.8%). Moreover, the result statistics indicated that there were PCR inhibition in stools, rectal swabs and throat swabs specimens with inhibition ratio from 1.8% to 3.4%. The inhibition ratio of these samples showed the importance of IC when PCR was used to detect the RNA of EV71, CA16 or other enterovirus. Conclusion] As a result of its high specificity, sensitivity and avoiding false negative results by using an internal control , the assay is suitable for rapid clinical diagnosis of EV71 and CA16.
%K EV71<br>内标
%K 多重荧光RT-PCR
%K 柯萨奇病毒A16型
%K 检测
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=D555E59C0E0E8A6B7DDECF5FFBCCFBB8&yid=DE12191FBD62783C&vid=2A3781E88AB1776F&iid=CA4FD0336C81A37A&sid=10F298ED9F164662&eid=DBF54A8E2A721A6D&journal_id=0001-6209&journal_name=微生物学报&referenced_num=1&reference_num=23