%0 Journal Article
%T Construction of infectious molecular clone of Duck hepatitis virus type 1 CL strain<br>1型鸭肝炎病毒CL株感染性分子克隆的构建
%A Kexiang Yu
%A Xiuli M
%A Yufeng Li
%A Jing Wu
%A Shuqian Lin
%A Yifei Jiang
%A Bing Huang
%A <br>于可响
%A 马秀丽
%A 李玉峰
%A 吴静
%A 林树乾
%A 姜亦飞
%A 黄兵
%J 微生物学报
%D 2009
%I 
%X Abstract: Objective] To construct an infectious clone for studying functions of duck hepatitis virus(DHV) type1 genome by reverse genetic technique. Methods] Three fidelity DNA fragments covering the full genome of DHV type1 CL strain were amplified by RT-PCR, and inserted into pBR322 vector, resulting in the full-length cDNA clone BR-CL. The in vitro-transcribed RNA from BR-CL was transfected into duck embryo renal cells and the rescued virus was identified using RT-PCR, indirect immunofluorescence assay and colloidal gold immunoelectron microscopy after six generations. After inoculating the rescued virus into SPF chick embryos, embryo death and pathological changes were observed. Results] The results of RT-PCR, indirect immunofluorescence assay and immunoelectron microscope showed that infectious virus was rescued. After inoculating into SPF chick embryos, the rescued virus was able to kill embryos with pathogenic changes. Conclusion] This is the first report on generation of infectious cDNA clone of DHV, which provides a valuable platform for further research on functions of DHV genome.
%K Keywords: Duck hepatitis virus type1
%K full-length cDNA clone
%K rescued virus<br>关键词：1型鸭肝炎病毒
%K 全长cDNA克隆
%K 拯救病毒
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=DF9F916E9E9E85897EA61E43D337A769&yid=DE12191FBD62783C&vid=2A3781E88AB1776F&iid=59906B3B2830C2C5&sid=6F76CBC90E48A1BC&eid=ED2274D65CDCCA90&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=10