%0 Journal Article
%T Selection of Yeast High in Lipids-yield by Fatty Acid Synthetase Inhibitor and Phosphoric Acid-vanillin Reaction<br>利用脂肪酸合成酶抑制剂和磷酸香草醛反应筛选高产油脂酵母菌
%A LI Ren-Min
%A WANG Ju-Fang
%A MA Shuang
%A YAN Ya-Ping
%A LI Wen-Jian
%A <br>李仁民
%A 王菊芳
%A 马 爽
%A 严亚平
%A 李文建
%J 微生物学报
%D 2008
%I 
%X Rhodotorula glutinis (Fresenius) Harrison was irradiated with 80 MeV/u carbon ions. primary selection of yeast high in lipids-yield was performed with culture medium supplemented cerulenin, an inhibitor of fatty acid synthetase (FAS). The quantity of the lipids of selected strains was analyzed by phosphoric acid-vanillin reaction and traditional methanol-chloroform extraction methods. The results showed that the growth of Rhodotorula glutinis (Fresenius) could be inhibited efficiently by cerulenin. The inhibition rate was up to 98% at cerulenin concentration of 8.96×10-6 mol/L, which was considered suitable for mutant selection. Through the phosphoric acid-vanillin reaction, it was found that the quantity of the lipids was a positive linear relation with the OD at 530 nm. The positive mutant rate of primary selection reached to 65%. With the advantage of rapid and easy to perform, it can be considered as a good method to select yeast high in lipid-yield. By these methods, 2 mutant strains with lipid yield improved 90% compared with control was selected out.
%K Microbial lipids
%K Fatty acid synthetase (FAS)
%K Cerulenin
%K Phosphoric acid-vanillin reaction<br>微生物油脂
%K 脂肪酸合成酶
%K Cerulenin
%K 磷酸香草醛反应
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=BF099C4431E8007CA56743DD65732368&yid=67289AFF6305E306&vid=6209D9E8050195F5&iid=E158A972A605785F&sid=89906B64FE741834&eid=C50DA610856963EE&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=0