%0 Journal Article
%T Analysis of tdh gene and its adjacent loci of Vibrio parahaemolyticus isolates from seafoods<br>副溶血弧菌海产品分离株tdh基因及其临近区域结构分析
%A Shuna Wang
%A Khamphouth VONGXAY
%A Biao Shen
%A Xiangyang Zhou
%A Peijie Jin
%A Jianshun Chen
%A Weihuan Fang
%A <br>王淑娜
%A Khamphouth VONGXAY
%A 沈飚
%A 周向阳
%A 金培婕
%A 陈健舜
%A 方维焕
%J 微生物学报
%D 2009
%I 
%X Abstract: Objective] To analyze the structural characteristics of tdh gene and its adjacent loci of Vibrio parahaemolyticus isolates from seafoods. Methods] Long distance PCR and genome walking were used to amplify the DNA sequences flanking the tdh gene, and the sequences were analyzed by blastn against the NCBI database. Results] The genetic structure of tdh-adjacent loci (VPA1312-VPA1327) from isolate ZS34 was similar to that of the reference strain RIMD2210633, with the nucleotide identity of 98.3%. The tdh gene of isolates FJ14 and WZ64 was located in the loci different from the reference strain and ZS34, and showed high nucleotide similarity to tdh3 gene. In the genome of isolate FJ14, tdh was 15kb away from the trh-ure cluster, with IS-like elements and transposase genes inserted therein. Isolate WZ64 lacked trh gene, but also harbored IS-like elements upstream of tdh. Conclusion] The tdh-adjacent loci of V. parahaemolyticus seafood isolates exhibit high diversity, an additional evidence of lateral gene transfer in this particular species.
%K Key words: Vibrio parahaemolyticus
%K tdh gene
%K IS-like elements
%K Lateral gene transfer<br>关键词：副溶血弧菌，tdh
%K 基因，类插入序列，基因水平转移
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=9DB19A954340D92697ACDC46D7EDF91B&yid=DE12191FBD62783C&vid=2A3781E88AB1776F&iid=59906B3B2830C2C5&sid=24F102A8BA6EDE6E&eid=39AB3D3C12DB6AFD&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=22