%0 Journal Article
%T Isolation and identification of a novel cis-epoxysuccinate hydrolase- producing Bordetella sp. BK-52 and optimization of enzyme production<br>产顺式环氧琥珀酸水解酶的博德特氏菌BK-52的筛选、鉴定及其产酶条件优化
%A Haifeng Pan
%A Zhipeng Xie
%A Wenna Bao
%A Jianguo Zhang
%A <br>潘海峰
%A 谢志鹏
%A 鲍文娜
%A 张建国
%J 微生物学报
%D 2008
%I 
%X Objective] To isolate and identify a novel strain with cis-epoxysuccinate hydrolase (CESH) activity and to optimize its enzyme production. Methods] The isolated strain was identified by electron microscopy, Biolog gram negative (GN) test, G+C (guanine plus cytosine) content measurement and 16S rDNA sequence. The purified enzymatic biotransformed product was identified by IR, 1H-NMR, 13C-NMR, MS and optical rotation analysis. Then the fermentation conditions for CESH production were optimized. Results] A novel CESH-producing strain was isolated for biotransforming cis-epoxysuccinate to D(-)-tartaric acid. It was assigned to genus Bordetella and named Bordetella sp. BK-52. The optimal conditions were found to be 30°C, pH 7.0, fermentation time 36 h, car-bon source of saccharose, inorganic nitrogen source of ammonium sulfate and enzyme inducer of disodium cis-epoxysuccinate. Under these conditions, the maximum CESH activity reached 764 U/g biomass. Conclusion] The isolated Bordetella sp. BK-52 provided a new alternative for biosynthesis of D(-)-tartaric acid from cis-epoxysuccinate.
%K Bordetella
%K cis-epoxysuccinate hydrolase
%K D(-)-tartaric acid
%K Isolation
%K Identification<br>博德特氏菌
%K 顺式环氧琥珀酸水解酶
%K D(-)-酒石酸
%K 筛选
%K 鉴定
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=988CEA641AC4DFE7DC6C1A43CB179943&yid=67289AFF6305E306&vid=B6DA1AC076E37400&iid=5D311CA918CA9A03&sid=6A12B9FCEF71AE29&eid=5A9F0976AE79CB6F&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=17