%0 Journal Article
%T Construction and the immunogenicity of the recombinant Modified Vaccinia Virus Ankala co-expressing ORF4、ORF5 and ORF6 genes of Porcine Reproductive and Respiratory Syndrome Virus NJ-a strain<br>共表达猪呼吸与繁殖障碍综合征病毒NJ-a株ORF4、ORF5与ORF6基因重组改良型痘苗病毒安卡拉株的构建
%A ZHENG Qi-sheng
%A BI Zhi-xiang
%A LI Peng
%A CHEN De-sheng
%A CHEN Pu-yan
%A <br>郑其升
%A 毕志香
%A 李鹏
%A 陈德胜
%A 陈溥言
%J 微生物学报
%D 2007
%I 
%X To develop investigate the recombinant MVA(rMVA) vaccines against PRRSV infection, the ORF4, ORF5 and ORF6 of PRRSV NJ-a strain were subcloned into the MVA transfer vector p II LR and the resultant recombinant vector was called p II LR-ORF4/ORF5/ORF6. The rMVA was generated by transfecting MVA-infected BHK-21 cells with the recombinant vector and screened by plaque purification after X-gal staining. After six rounds of purification, insertion of PRRSV GP4, GP5 and M genes into the MVA genome was confirmed by PCR analysis and expression of the three proteins was identified by Western-blot and IFA. Each of the tested mice was inoculated with 5 x 10(5) TCID(50)/mouse of the rMVA-GP4/GP5/M and boosted 3 weeks later. Neutralization assay showed that PRRSV-specific neutralizing antibodies were detectable at 3 weeks and reached the highest titer (2(5)) by 8 weeks after the primary vaccination, which maintained stable until the end of the experiment. The significant lymphocyte proliferation responses were also observed in mice immunized with rMVA-GP4/GP5/M. These results indicate the rMVA co-expressing PRRSV ORF4, ORF5 andORF6 genes may be an attractive candidate vaccine for preventing PRRSV infection.
%K PRRSV
%K MVA
%K GP4
%K GP5 and M proteins
%K co-expression<br>猪繁殖与呼吸综合征病毒
%K 修饰的痘苗病毒安卡拉株
%K GP4、GP5与M蛋白
%K 共表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=35C99FDF0E692359&yid=A732AF04DDA03BB3&vid=F4B561950EE1D31A&iid=0B39A22176CE99FB&sid=3622B70F9C54A9CC&eid=31125890FF093250&journal_id=0001-6209&journal_name=微生物学报&referenced_num=4&reference_num=17