%0 Journal Article
%T Construction and Functional Analysis of The pepB Gene Disruptant in Aspergillus niger<br>黑曲霉pepB基因缺失菌株的构建及其功能分析
%A SUN Jing
%A LI Jing-Peng
%A WANG Ao-Quan
%A TANG Guo-Min
%A WANG Hua-Ming
%A <br>孙晶
%A 李景鹏
%A 王敖全
%A 唐国敏
%A 王华明
%J 微生物学报
%D 2004
%I 
%X An integration plasmid pMW1-pepB for the pepB gene disruption in Aspergillus was constructed. The plasmid contained the pepB gene upstream (P) 1.4kb and downstream (T) 1.3kb homologous fragments with insertion of the expression unit of the hygromycin resistance gene (hph) between them. P and T DNA fragments were synthesized by PCR from Aspergillus niger chromosomal DNA. The integration plasmid was digested with the Hpa I restriction enzyme, the resultant 4.2kb linear fragment was introduced into the Aspergillus niger strain GICC2773 which expressing the glucoamylase/laccase fusion protein by PEG-mediated transformation. 62 Hygromycin resistance transformants were screened, and from them one strain named pepB29 was identified to be the pepB disruptant by PCR analysis. Data of functional assay of the pepB29 strain indicated that the disruption of the pepB gene secreted reduced acid proteolytic activity, and improved the heterologous protein laccase production.
%K Aspergillus niger
%K pepB gene deletion
%K Homologous recombination
%K Heterologous protein<br>黑曲霉
%K pepB基因缺失
%K 同源重组
%K 外源蛋白
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=2D20BB825BA54BE5&yid=D0E58B75BFD8E51C&vid=1AE5323881A5ECDC&iid=B31275AF3241DB2D&sid=34A7AB0452E6AF23&eid=BDEE8BA20F4733DB&journal_id=0001-6209&journal_name=微生物学报&referenced_num=7&reference_num=12