%0 Journal Article
%T Isolation and Identification Alicyclobacillus from Concentrated Apple Juice processing<br>浓缩苹果汁生产过程中脂环酸芽孢杆菌的分离及初步鉴定
%A CHEN Shi-Qiong HU Xiao-Song
%A SHI Wei-Ni DONG Jian-Cheng WANG Jian
%A <br>陈世琼
%A 胡小松
%A 石维妮
%A 董建成
%A 王健
%J 微生物学报
%D 2004
%I 
%X The forty-five strains of bacteria have been isolated from concentrated apple juice. All of them can grow at K agar (pH 4.0), but can't grow at LB palate (pH 7.0). By using heat resistance test, 38 strains of these bacteria could be classified into 3 groups according to their spore-forming time. 33 strains of them had the same spore-forming time as DSM 3922, which could produce spores within 48 h when cultivated at 45 degrees C; 3 strains of them could grow very fast and produced spores within 17 h; and 3 strains of them produced spores 48h later. Based on the results of 16S rDNA PCR-RFLP analysis, 7 strains of unknown, probably new species and 5 reference strains were selected for 19 phenotypic characteristics test and the result were analyses by UPGMA. The results of phenotypic characteristics test had good agreement with 16S rDNA PCR-RFLP analyses, that the 7 strains were different from all the 5 reference strains, they are new species of Alicyclobacillus.
%K Alicyclobacillus
%K Isolation
%K Phenotypic characteristics
%K Concentrated Apple Juice<br>脂环酸芽孢杆菌
%K 浓缩苹果汁
%K 分离
%K 表型特征
%K 聚类分析
%K 鉴定
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=E54296A33F45D343&yid=D0E58B75BFD8E51C&vid=1AE5323881A5ECDC&iid=B31275AF3241DB2D&sid=43AADF4B53A8BF6F&eid=C79A3F06E2AC5B9E&journal_id=0001-6209&journal_name=微生物学报&referenced_num=7&reference_num=15