%0 Journal Article
%T Effect of clpE gene deletion on virulence of Streptococcus pneumoniae<br>clpE基因缺失对肺炎链球菌毒力的影响
%A Qun Zhang
%A Nanlin Yin
%A Wenchun Xu
%A Hong Wang
%A Dan Pang
%A Xiaoliang Yang
%A Yibing Yin
%A Xuemei Zhang
%A <br>张群
%A 尹楠林
%A 胥文春
%A 王虹
%A 庞丹
%A 杨晓亮
%A 尹一兵
%A 张雪梅
%J 微生物学报
%D 2009
%I 
%X Objective] To study the effect of clpE gene deletion on the virulence of Streptococcus pneumoniae. Methods] The clpE-deficient strain was constructed by LFH-PCR and identified by PCR and sequencing. The impact of clpE mutant on the virulence of S. pneumoniae was evaluated in a mouse model. In addition, we also studied the effect of clpE mutant on adherence and invasion of host cells. Real time RT-PCR was used to measure the mRNA expression levels of autolysin A, pneumococcal surface adhesion A, pneumolysin, pneumococcal surface protein A and neuraminidase Results] The clpE gene was replaced completely by erm cassette. Mice virulence experiments showed that the median lethal time of the wide-type was 54 h, whereas that of clpE mutant was 21d (P<0.01). Cell culture infection experiments indicated that adherence and invasion of clpE mutant were strongly reduced (P<0.05). The expression of virulent factors in clpE mutant was lower than that of the wild-type (P<0.05). Conclusion] ClpE is involved in virulence by modulating the expressions of virulence factors.
%K streptococcus pneumoniae
%K virulence factor
%K real time RT-PCR
%K bacterial adhesion
%K clpE gene<br>肺炎链球菌
%K 毒力因子
%K 实时荧光定量PCR
%K 细菌粘附
%K clpE基因
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=3A634E26F78B623BADD1401872724D39&yid=DE12191FBD62783C&vid=2A3781E88AB1776F&iid=0B39A22176CE99FB&sid=FD7C952458BFB5D8&eid=9CA95D22FC1D537C&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=17