%0 Journal Article
%T Genetic Transformation of Buckwheat (Fagopyrum esculentum Moench) with AtNHX1 Gene and Regeneration of Salt-tolerant Transgenic Plants<br>AtNHX1基因对荞麦的遗传转化及抗盐再生植株的获得
%A CHENG Li-Hong
%A ZHANG Bo
%A XU Zi-Qin
%A <br>陈利红
%A 张波
%A 徐子勤
%J 微生物学报
%D 2007
%I 
%X The Arabidopsis thaliana tonoplast Na+/H+ antiporter gene, AtNHX1, was transferred into buckwheat by Agrobacterium-mediated method. Transgenic buckwheat plants were regenerated and selected on MS basal medium supplemented with 2.0mg/L 6-BA, 1.0mg/L KT, 0.1mg/L IAA, 50mg/L kanamycin and 500mg/L carbenicillin. 426 seedlings from 36 resistant calli originated from 864 explants (transformed about at 4.17 percentage) exhibited resistance to kanamycin. The transformants were confirmed by PCR, Southern blotting, RT-PCR and Northern blotting analysis. After stress treatment for 6 weeks with 200mmol/L NaCl, transgenic plants survived, while wild-type plants did not. After 3 days of stress treatment through different concentrations of NaCl, transgenic plants accumulated higher concentration of Na+ and proline than the control plants. However, the K+ concentration of transgenic plants declined in comparison with the control plants. Moreover, the rutin content of the roots, stems and leaves of transgenic buckwheat increased than those of the control plants. These results showed that it could be possible to improve the salt-tolerance of crops with genetic technology.
%K Fagopyrum esculentum Moench
%K Na+/H+ antiporter
%K salt tolerance
%K rutin
%K HPLC<br>荞麦
%K Na+
%K /H+反向转运蛋白，
%K 耐盐性，
%K 芦丁，
%K 高效液相色谱
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=B1746CC9E558CBDA3EEC26846EE17F6D&yid=A732AF04DDA03BB3&vid=EA389574707BDED3&iid=CA4FD0336C81A37A&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=0