%0 Journal Article
%T Cryopreservation of Cyclamen persicum Mill. Callus<br>仙客来愈伤组织的超低温保存
%A Fuhua Bian
%A Xueqin Gong
%A Cuirong You
%A Caixia Zheng
%A Funing Qu
%A <br>卞福花
%A 龚雪琴
%A 由翠荣
%A 郑彩霞
%A 曲复宁
%J 微生物学报
%D 2008
%I 
%X In this paper we studied cryopreservation of Cyclamen persicum Mill. callus to avoid variations produced by sub-culture. The callus in the logarithmic phase after sub-culture were used for experiments. Firstly, the callus were pre-cultured in culture-medium containing 4%, 6% or 8% sucrose for different time periods, transferred to different cryoprotectants to directly cryopreserve or incubated for 2 hours at -20°C, then submersed in liquid nitrogen, lastly thawed rapidly in a waterbath at 37°C, and washed with liquid culture-medium containing the corresponding concentration of sucrose. Cell survival rate was computed after stained by Neutral Red, and SPSS 13.0 software was used for statistical analysis. The results showed that sucrose concentration, pre-culture time, cryoprotectants had various impacts on cell survival rate. We have developed a simple but effective protocol for the cryopreservation of callus of C. persicum. Of the different protocols tested, 4%sucrose, pre-culturing for 3 days, No. 9 cryoprotectant and freezing directly after 30 minutes at 0°C results in the highest cell survival rate.
%K Cyclamen persicum Mill
%K callus
%K cryopreservation
%K survival rate<br>仙客来
%K 愈伤组织
%K 低温保存
%K 存活率
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=58F2B6E37E8ADEEDEA14B9834D6A86AD&yid=67289AFF6305E306&vid=B91E8C6D6FE990DB&iid=38B194292C032A66&sid=9B1D77939DDB4B89&eid=37F781FD8E744761&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=0