%0 Journal Article
%T Screening and characterization of antibacterial and cytotoxic marine bacteria associated with Karlodinium micrum<br>微小卡罗藻（Karlodinium micrum）共附生微生物抗菌与细胞毒活性
%A Peng Zhu
%A Yurong You
%A Jiaojiang Chu
%A Haixiao Jin
%A Xiaojun Yan
%A <br>朱鹏
%A 游玉容
%A 褚椒江
%A 金海晓
%A 严小军
%J 微生物学报
%D 2010
%I 
%X Abstract: Objective] Antibacterial and cytotoxic activities were screened for marine bacteria which have been isolated from Karlodinium micrum, in order to obtain potential strains with antibacterial and cytotoxic activities. Methods] In total 38 bacteria isolated from Karlodinium micrum were screened by agar-screening and MTT methods. The 16S rRNA genes were amplified from the genome DNA of those bacteria positive for both antibacterial and cytotoxic activities, which were cloned into pMD18-T vector for sequencing analysis. Results] Twenty-five isolates had antimicrobial activity and 5 isolates (W-14-2, W-2-2, W-12, E-8-2 and W-4) had cytotoxicity. Molecular phylogenetic analysis of marine bacteria with cytotoxicity based on 16S rRNA sequences indicated that they exhibited the highest similarity (98%, 99%, 99%, 98% and 99%, respectively) to the 16S rRNA fragments of Alteromonas alvinellae, Stappia aggregata, Pelagibaca bermudensis, Marinobacter kribbensis and Maribacter dokdonensis. Conclusion] The bacteria with bioactivity in Karlodinium micrum were abundant. We obtained five strains positive for both antibacterial and cytotoxic activities, which provide a clue to understanding the mechanism of toxin biosynthesis in Karlodinium micrum based on epiphytic and endophytic bacteria.
%K Keywords: Epiphytic and endophytic bacteria of Karlodinium micrum
%K antibacterial activity
%K cytotoxicity
%K 16S rRNA<br>关键词：卡罗藻共附生微生物
%K 抗菌活性
%K 细胞毒活性
%K 16S
%K rRNA
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=39BA400C99C58CCAF3D02713AAB9C303&yid=140ECF96957D60B2&vid=771152D1ADC1C0EB&iid=5D311CA918CA9A03&sid=42AB3C691163F5B1&eid=208AC7DC28BD1EC6&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=0