%0 Journal Article
%T Sequencing and analysis of Vibrio cholerae typing phage VP3 genome<br>霍乱弧菌分型噬菌体VP3基因组序列的测定与分析
%A ZHAO Ying-wei
%A WANG Duo-chun WANG Min LI Yan-ping
%A KAN Biao
%A <br>赵英伟
%A 王多春
%A 汪敏
%A 李燕萍
%A 阚飙
%J 微生物学报
%D 2005
%I 
%X DNA sequence and the genome of phage VP3(a typing phage of V. cholera) were analyzed. A random library of VP3 DNA was constructed by shot-gun library method. The VP3 genome sequence was assembled with contigs sequences, the gaps between different contigs were filled with sequencing data from primer walking. ORFs were predicted; Phylogeny of DNA polymerase sequences was analyzed to determine the class of VP3; The activity of putative promoter genes were analyzed using %lacZ% report system. VP3 genome is a 39504bp of circular double-stranded DNA. Twenty-seven out of forty-nine putative ORFs were annotated; twenty gene products were homologous with T7-like phages, including DNAP, DNA replicative protein, capsid, tail tubular, tail fiber protein, and DNA packaged protein. The activity of the putative promoter regions was confirmed through cloning those regions to LacZ-fuse plasmid pRS1274 and analysis of the expression of beta galactosidase. The complete genomic sequence of VP3 and phylogenetic tree analysis suggests VP3 is a member of T7 phage family.
%K Vibrio cholerae
%K Phage VP3
%K Genome<br>霍乱弧菌
%K 噬菌体VP3
%K 基因组
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=6531B3A738DCFE85&yid=2DD7160C83D0ACED&vid=94E7F66E6C42FA23&iid=B31275AF3241DB2D&sid=0075485171F295AA&eid=466EAEA150F7AE77&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=12