%0 Journal Article
%T Study on transformation of P-dissolving Penicillium oxalicum P8 with double-marker vector expressing green fluorescent protein and hygromycin B resistance<br>绿色荧光蛋白和潮霉素抗性双标记载体转化草酸青霉菌P8的研究
%A ZHANG Lei
%A FAN Bing-quan
%A HUANG Wei-yi
%A <br>张磊
%A 范丙全
%A 黄为一
%J 微生物学报
%D 2005
%I 
%X P-dissolving Penicillium oxalicum P8 was isolated previously in this lab which has a considerable ability to dissolve many kinds of inorganic phosphorus and improve crop growth. In order to study rhizosphere colonization of plants by Penicillium oxalicum P8, protoplasts were transformed with a double-marker expression vector of green fluorescent protein and hygromycin B resistance. Some transformants were selected which expressed both the GFP and hygromycin B phosphotransferase and did not show significant morphological or physiological differences as compared to wild-type strain. Southern blot analysis confirmed the heterogeneous genomic integration of the vector DNA into the transformants.
%K Penicillium oxalicum P8
%K Transformation
%K Green fluorescent protein gene
%K Hygromycin B phosphotransferase gene(hph)<br>草酸溶磷青霉菌P8
%K 转化
%K 绿色荧光蛋白(GFP)
%K 潮霉素磷酸转移酶基因(hph)
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=F43998EDEBD9B8C5&yid=2DD7160C83D0ACED&vid=94E7F66E6C42FA23&iid=B31275AF3241DB2D&sid=043C7D0F3F6AC1B3&eid=C3565B78E68229C7A7252C54B9050A85&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=16