%0 Journal Article
%T Real time PCR quantification of ammonia-oxidizing bacteria in aerobic granular sludge and activated sludge influenced by pentachlorophenol<br>荧光定量PCR监测五氯酚对好氧颗粒污泥和活性污泥中氨氧化细菌数量的影响
%A LI Guang-wei
%A LIU He
%A ZHANG Feng
%A DU Guo-cheng
%A CHEN Jian
%A <br>李光伟
%A 刘和
%A 张峰
%A 堵国成
%A 陈坚
%J 微生物学报
%D 2007
%I 
%X The V2 region of the 16S ribosomal DNA of the ammonia-oxidizing bacteria (AOB) was amplified directly from the environmental sample by using the specific PCR primers. The purified PCR product was cloned into T-vector and was identified as 16S rDNA fragment of AOB by sequencing and Real-time PCR method. Then, the recombined plasmid was used as standard molecule sample in Real-time PCR for AOB quantification. The numbers of the AOB were monitored in samples of both aerobic granular sludge and activated sludge influenced by PCP by using Real-time PCR. The results showed that the numbers of AOB in aerobic granular sludge and activated sludge were 4.28 x 10(7) 5.44 x 10(6) cells/g dried sludge and 2.51 x 10(9) +/- 8.61 x 10(8) cells/g dried sludge without PCP in the reactors, respectively. With the increase of PCP concentration (from 0mg/L to 50mg/L), the numbers of AOB in both types of sludge had no obvious change( P > 0.05) . The numbers of AOB had no obvious correlation with ammonia removal ( P > 0.05) . The main effect of PCP on AOB in both types of sludge was to inhibit their metabolic activity.
%K Real-time PCR
%K Aerobic granular sludge
%K Activated sludge
%K Ammonia-oxidizing bacteria
%K Pentachlorophenol<br>荧光定量PCR
%K 好氧颗粒污泥
%K 活性污泥
%K 氨氧化细菌
%K 五氯酚
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=8C8B0130EC832FEB&yid=A732AF04DDA03BB3&vid=F4B561950EE1D31A&iid=CA4FD0336C81A37A&sid=58F693790F887B3B&eid=B0EBA60720995721&journal_id=0001-6209&journal_name=微生物学报&referenced_num=1&reference_num=11