%0 Journal Article
%T Generation of recombinant vaccinia virus expressing attachment glycoprotein of Nipah virus<br>表达尼帕病毒G囊膜糖蛋白重组牛痘病毒的研究
%A WANG Xi-jun
%A WANG Qing-hua
%A GE Jin-ying
%A HU Sen
%A BU Zhi-gao
%A <br>王喜军
%A 王清华
%A 葛金英
%A 胡森
%A 步志高
%J 微生物学报
%D 2006
%I 
%X The mammalian condon optimized G gene was synthesized by over-lapping PCR and used to generate recombinant vaccinia virus, rWR-NiV-G. The expression of Nipah virus G protein in rWR-NiV-G infected HeLa cells was confirmed by western-blot with NiV G protein specific mouse antiserum generated by DNA immunization.The recombinant G protein showed sensitive and specific antigenic reaction to rabbit serum anti-Nipah virus in indirect florescence. Syncytium formation was induced in BHK cells by rWR-NiV-G infection following NiV F protein expressing plasmid pCAGG-NiV-F transfection. Immunization with rWR-NiV-G elicited G protein specific antibody responses in mice. The prokaryotic expressing G protein fragment showed sensitive and specific antigenic reaction to NiV G protein specific antibody from rWR-NiV-G immunized mice serum in indirect ELISA. Furthermore, the G protein specific antibodies could neutralize the infectivity of the recombinant Vesicular Stomatitis Virus pseudotype VSVAG * F/G, in which the VSV envelope protein G gene was replaced with the green fluorescent protein gene (VSVAG * G, Whitt MA) and complemented with Nipah virus F and G glycoprotein expressed in transient (VSVAG * F/G).The results here demonstrated the G protein expressed by rWR-NiV-G keeps native immunogenicity and biological activity. The recombinant virus could be promising vaccine strategy for the prevention of Nipah virus.
%K Nipah virus
%K Attachment glycoprotein
%K Recombinant vaccinia virus<br>尼帕病毒
%K G蛋白
%K 重组牛痘病毒
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=4B2280C086BBAB5D&yid=37904DC365DD7266&vid=D997634CFE9B6321&iid=E158A972A605785F&sid=0636354D8CF77519&eid=4C62402D70501D0B26213ADA963E22ED&journal_id=0001-6209&journal_name=微生物学报&referenced_num=1&reference_num=15