%0 Journal Article
%T Cloning and Expression of gB Gene of Infectious Laryngotracheitis Virus in M.Smegmatis<br>鸡传染性喉气管炎病毒gB基因的克隆及其在耻垢分枝杆菌中的表达
%A Hai-Zhou Zheng
%A Hong Yang
%A Jia-Ning Bai
%A Bao-Hua Zhao
%A <br>郑海洲
%A 杨虹
%A 柏佳宁
%A 赵宝华
%J 微生物学报
%D 2004
%I 
%X Firstly, the complete gB gene of a domestic isolation stain were amplified by PCR, and the 2.6 kb gene fragment was obtained. Then the recombinant plasmid pY-alpha-gB was constructed by cloning PCR product into pY-alpha vector, and the shuttle expression plasmid pR-alpha-gB was constructed by cloning the hsp-alpha-gB gene into the downstream sequences of pRR3 vector. The recombinant plasmid was identified by restriction enzyme digestion and the sequence analysis, which was electrophoreted into M. smegmatis mc2 155. At last, the expressed gB proteins were successfully detected by ELISA and Western blot, which seems to be immunogenic crucially. The recombinant bacterial stain M. smegmatis mc2 155 (pR-alpha-gB)could protect SPF chick embryo from one lethal dose of ILTV.
%K Polymerase chain reaction
%K Cloning
%K Expression
%K The shuttle expression vector<br>聚合酶链式反应
%K 克隆
%K 表达
%K 穿梭表达载体
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=59EF9094109A7FCA&yid=D0E58B75BFD8E51C&vid=1AE5323881A5ECDC&iid=B31275AF3241DB2D&sid=CE16DE2ABCD4D365&eid=07034C6B9EA4A53C&journal_id=0001-6209&journal_name=微生物学报&referenced_num=4&reference_num=9