%0 Journal Article
%T High-titer preparation of HIV-1-based defective lentivector and it mediated efficient gene transfer<br>HIV-1缺损慢病毒载体的高滴度制备及其介导的高效基因转移
%A ZENG Ling-bing
%A ZHANG Lin
%A MENG Yan
%A Yuanan Lu
%A YE Lin-bai
%A <br>曾令兵
%A 张林
%A 孟彦
%A Yuanan Lu
%A 叶林柏
%J 微生物学报
%D 2007
%I 
%X Lentivectors have drawn considerable attention recently and become important delivery vehicles for gene transfer manipulation. By Transiently co-transfecting 293T packaging cells with three DNA plasmids system encoding lentivector constituents, a protocol for bulky preparation of human immunodeficiency virus type-1 (HIV-1)-based defective lentivector with high titer has been established. Transient co-transfection of 293T packaging cells resulted in production of high-titer vector (1.1×107IU/ml), which can be further concentrated over 100-fold through a single step centrifugation. The vector was capable of efficiently transducing a variety of cells from both primate and non-primate sources, including of human T-lymphoblastoid cell line. Long-term culture of vector transduced cells showed a stable expression of foreign gene over 18 months detected by RT-PCR. Assessment of potential generation of replication-competent virus revealed no detection of p24 antigen protein or infectious particles in vector-transduced cells.
%K HIV-1-based Lentivector
%K preparation
%K high titer
%K gene transfer
%K efficiency<br>HIV-1慢病毒载体
%K 制备
%K 高滴度
%K 基因转移
%K 效率
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=ED2CC2FD33A6F13B3D93A3C34742478F&yid=A732AF04DDA03BB3&vid=F4B561950EE1D31A&iid=B31275AF3241DB2D&sid=6B1D468AD7B8ADD8&eid=4CB3DBBCE4B39661&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=13