%0 Journal Article
%T Expression and Antigenic Characterization of the Epitope-G1 of Bovine Ephemeral Fever Virus Glycoprotein in Pichia pastoris<br>牛流行热病毒糖蛋白G1抗原表位在毕赤酵母中的表达和抗原性鉴定
%A ZHENG Fu-Ying
%A LIN Guo-Zhen
%A QIU Chang-Qing
%A YUAN Kui-Zhang
%A SONG Jun-Ying
%A <br>郑福英
%A 蔺国珍
%A 邱昌庆
%A 原魁章
%A 宋军英
%J 微生物学报
%D 2007
%I 
%X The epitope-G1 gene, cloned from the pMD-G plasmid including G protein gene of bovine ephemeral fever virus, was subcloned into expression vector pPIC9K to construct pPIC9K-G1 recombinant plasmid successfully, and the recombinant plasmid linearized was transformed into Pichia pastoris GS115 by electroporation. The recombinant Pichia pastoris strains were screened by G418 and PCR, and induced by methanol. The expressed products were analyzed by SDS-PAGE, deglycosylation, Western blot, ELISA, immunizing rabbits and specificity experiments. The results indicated that the gene was expressed successfully in GS115 and glycosylated moderately, and the target protein had nicer biological activity and specificity. The protein is able to be used as coating antigen to develop ELISA Kit for diagnosing bovine ephemeral fever.
%K Bovine ephemeral fever virus
%K Epitope-G1
%K Pichia pastoris
%K Expression
%K Characterization<br>牛流行热病毒，G1抗原表位，毕赤酵母，表达，鉴定
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=973DA75DF405D9D50A9875F1EB427131&yid=A732AF04DDA03BB3&vid=339D79302DF62549&iid=94C357A881DFC066&sid=9854BDFCD82ADBE1&eid=C2093132FB6AB303&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=0