%0 Journal Article
%T Construction of an engineering strain producing alkaline pectate lyase with pHSh<br>利用温控载体构建碱性果胶酯裂解酶工程菌
%A ZHUGE Bin
%A DU Guo-cheng
%A SHEN Wei
%A ZHUGE Jian
%A CHEN Jian
%A TANG Xue-ming
%A WANG Chun
%A <br>诸葛斌
%A 堵国成
%A 沈微
%A 诸葛健
%A 陈坚
%J 微生物学报
%D 2006
%I 
%X The structure gene PL from Bacillus subtilis WSHB04-02 encoding pectate lyase was amplified by PCR.The pET22b( ) vector,with leader sequence PelB,harboring PL gene was constructed.From pET22b( )PL,the fragment of PL and leader sequence PelB was amplified by PCR together,which was transformed into E.coli JM109.The expression of PL in E.coli JM109 was not evidently different from E.coli BL21DE3(pET22b( )PL) which promoter is T7.SDS-PAGE analysis showed that the molecular weight of expressed recombinant PL was about 43kDa which was the same as calculated value.The results indicated the expression of pHsh PL in E.coli JM109,Hsh as a promoter,was satisfied and low-cost.It is significant for large-scale fermentation of pectate lyase.
%K Pectate lyase
%K pHsh
%K Gene
%K Expression<br>碱性果胶酯裂解酶
%K pHsh
%K 基因
%K 表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=C8930AE1AE2D035A&yid=37904DC365DD7266&vid=D997634CFE9B6321&iid=E158A972A605785F&sid=E934BC2766053B28&eid=00520952CD4BF212&journal_id=0001-6209&journal_name=微生物学报&referenced_num=1&reference_num=9