%0 Journal Article
%T Polymorphism of Lactobacilli from fermented milk products in Northern Tibet<br>藏北地区传统发酵乳中乳杆菌的多样性分析
%A Ning Ju
%A Jiachao Zhang
%A Zhihong Sun
%A Wenjun Liu
%A YeJin
%A Heping Zhang
%A Tiansong Sun
%A <br>剧柠
%A 张家超
%A 孙志宏
%A 刘文俊
%A 靳烨
%A 张和平
%A 孙天松?
%J 微生物学报
%D 2009
%I 
%X Abstract: Objective] In order to study the Biodiversity of Lactobacilli which were from Northern Tibet in China, ERIC-PCR (enterobacterial repetitive intergenic consensus sequence polymerase chain reaction) technique was developed. Methods] Seventy seven Lactobacillus strains were isolated from traditional fermented milk products which are made by herd man families in Northern Tibet of China. By ERIC-PCR technique method, using NTSYS-pc2.1 software, we investigated the polymorphism and types of the strains. Results]The results suggested that the amplified bands of ERIC-PCR were clear and stable with high repetition and high polymorphisms. The cluster analysis showed that at level 0.73, 77 tested strains were clustered into 4 groups: A1: strains of Lactobacillus casei (35.06% of the strains), A2: strains of Lactobacillus fermentum (61.04% of the strains), A3: strains of Lactobacillus helveticus and A4: strains of Lactobacillus plantarum. The biodiversity of isolates from A1and A2 groups was further investigated. A1 could be divided into five genotypes at the similarity value of 80.0%. A2 could be divided into six genotypes at the similarity value of 84.0%. Conclusion]Using ERIC-PCR technique, the Lactobacilli from Northen Tibet could be distinguished at specices level. Results from this study showed that there was no positive relationship between the ERIC-PCR gene types and the origin of the strains.
%K 关键词：西藏
%K 传统发酵乳制品
%K 乳杆菌
%K 多样性
%K ERIC-PCR
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=AED4522A56DF3170848E0F6EA3B53D3B&yid=DE12191FBD62783C&vid=2A3781E88AB1776F&iid=708DD6B15D2464E8&sid=67E1C94EE625F186&eid=D9E4B4338A9AF29A&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=20