%0 Journal Article
%T Genetic Stability of A Recombinant Fowlpox Virus Co-expressing H5 HA and N1 NA Gene of Avian Influenza Virus<br>共表达禽流感病毒HA和NA基因重组禽痘病毒的遗传稳定性
%A QIAO Chuan-Ling
%A CHANG Hong-Tao
%A YU Kang-Zhen
%A CHEN Hua-Lan
%A <br>乔传玲
%A 常洪涛
%A 于康震
%A 陈化兰
%J 微生物学报
%D 2004
%I 
%X A recombinant fowlpox virus (rFPV) containing H5 HA and N1 NA gene was serially passaged to passage 25 on specific-pathogen-free (SPF) chicken embryo fibroblast monolayer. The HA gene and NA gene from passage 5,15 and 25 were amplified by polymerase chain reaction (PCR) and sequenced. SPF chickens were vaccinated with the recombinant viruses of three passages. The results demonstrated that specific fragments of HA and NA were cloned, identified and sequenced from the three passages, respectively, and there were no change in the amino acid sequences. The virulence and immune efficacy of the recombinant virus was not changed with the passage of the virus. These three passages of recombinant virus could provide complete protection against challenge with homologous HPAIV strain and is genetically stable for at least 25 passages.
%K Avian Influenza
%K Recombinant Fowlpox Virus
%K Genetically Stability<br>禽流感
%K 重组禽痘病毒
%K 遗传稳定性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=4BB6772DCC9D1AC2&yid=D0E58B75BFD8E51C&vid=1AE5323881A5ECDC&iid=94C357A881DFC066&sid=710C005323C0774A&eid=2A22E972FD97071B&journal_id=0001-6209&journal_name=微生物学报&referenced_num=8&reference_num=6