%0 Journal Article
%T Screening and real-time quantitative detection for genomic signatures of Bacillus anthracis<br>炭疽芽胞杆菌染色体特异序列的筛选及实时定量检测
%A RONG Guang-hua
%A XIA Yi
%A ZHU Shi-ying
%A TONG Yi-min
%A QI Zhong-tian
%A <br>荣光华
%A 夏懿
%A 朱诗应
%A 童一民
%A 戚中田
%J 微生物学报
%D 2006
%I 
%X A total amount of 117 candidate chromosomal sequences were screened for the genomic signatures of Bacillus anthracis by a 2_step approach.Out of them, 19 genomic signatures sequences were selected,among which, 6 genomic signatures were competent as the target sequence of TaqMan real_time PCR method. With the most significant alignment and specificity, fragment C04, together with pagA gene and capB gene from virulence plasmids pX01 and pX02, was selected to establish a TaqMan real_time PCR assay. For each target sequence, as little as 10 to 100 copies per reaction could be detected. Twelve bacterial species including 7 from Bacillus cereus group which were closely related to Bacillus anthracis were used to test the specificity of this assay. Data revealed that the assay gained a 100% specificity. Further performance of the assay was assessed with 10 simulative contaminated environmental samples and 20 negative control environmental samples; all of the Bacillus anthracis contaminated samples gave strong positive signals while the control samples were negative. This specific and sensitive real_time PCR assay could be used in rapid confirmation or exclusion of potential bio_attacks and the diagnosis of anthrax.
%K Bacillus anthracis
%K Genomic signature
%K Real_time quantitative detection
%K PCR<br>炭疽芽胞杆菌
%K 染色体特异序列
%K 实时定量检测
%K PCR
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=B3D978F0D70C2DD7&yid=37904DC365DD7266&vid=D997634CFE9B6321&iid=B31275AF3241DB2D&sid=F3FF3E69C64937E9&eid=FE2777263ADBA5FE&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=22