%0 Journal Article
%T Analysis of the bacterial diversity in intestines of Hepialus gonggaensis larae<br>贡嘎蝠蛾幼虫肠道细菌多样性分析
%A Li Liu
%A Zhongkang Wang
%A Hewei Yu
%A Shijiang Chen
%A Guangfan Yan
%A Yuxian Xia
%A Youping Yin
%A <br>刘莉
%A 王中康
%A 俞和韦
%A 陈仕江
%A 阎光凡
%A 夏玉先
%A 殷幼平
%J 微生物学报
%D 2008
%I 
%X Objective] We investigated the intestinal microbial diversity in the larval gut of Hepialus gonggaensis, an economically important insect. Methods] We used morphological, physiological, chemotaxonomic characteristics and 16S rRNA analysis method, and the molecular method of PCR-DGGE (denaturing gradient gel electrophoresis) analysis based on the sequence of 16S rRNA V3 region gene. Results] By the traditional isolation method, 8 genera of bacteria were identified from 11 isolated bacterial populations. The dominant bacteria in intestine belonged to enterobacter. By 16S rRNA V3 region gene DGGE method, eleven distinct bands were obtained from 16S rDNA amplificons. The bands were purified, sequenced. The sequences aligned with GenBank database and showed that they were belonged to 8 different genera of bacteria. Phylogenetic analysis showed that the sequences of bacteria belonged to the Proteobacteria and Firmicutes. The most dominant bacteria group was Carnobacterium in the gut and Bacillus followed by it. The different patterns were observed in different instars larvae guts from DGGE profiles, which might be related to their physiological development stages. Conclusion] 8 genera were obtained from intestine of H. gonggaensis by traditional culturing method and 16S rDNA analysis method respectively, but the two groups were not exactly same, and the dominant group was different also. This suggested that a combination of molecular and traditional culturing methods can be used to analyze and monitor the diversity of intestinal microflora effectively, and that will give us more information of microorganism diversity.
%K Hepialus gonggaensis
%K 16S rRNA
%K normal isolation culture
%K denaturing gradient gel electrophoresis (DGGE)
%K specie diversity<br>贡嘎蝠蛾幼虫
%K 16S
%K rRNA
%K 常规分离培养
%K 变性梯度凝胶电泳
%K 群落多样性
%K 贡嘎
%K 蝠蛾幼虫
%K 肠道细菌
%K 多样性分析
%K intestines
%K bacterial
%K diversity
%K 信息
%K 微生物多样性
%K 肠道微生物
%K 分离法
%K 结合
%K 关系
%K 生理状态
%K 发育
%K 差异
%K 存在
%K 群的结构
%K 虫龄
%K 显示
%K 图谱
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=040FC1D3F3C0E96F57251EAD9EFF2FEF&yid=67289AFF6305E306&vid=B6DA1AC076E37400&iid=94C357A881DFC066&sid=984BD2F4D19B9D1C&eid=6837BC93241057EF&journal_id=0001-6209&journal_name=微生物学报&referenced_num=1&reference_num=18