%0 Journal Article
%T Construction and identification of recombinant BHV-1 expressing foot and mouth disease virus VP1 gene<br>表达O型口蹄疫病毒 VP1基因的重组病毒BHV-1的构建与鉴定
%A Xiangang Ren
%A Fei Xue
%A Yuanmao Zhu
%A Guanzhi Tong
%A Yanhui Wang
%A Junke Feng
%A Lichuang Zu
%A Jiao Li
%A Hongfei Shi
%A Yuran Gao
%A <br>任宪刚
%A 薛飞
%A 朱远茂
%A 童光志
%A 王延辉
%A 冯军科
%A 祖立闯
%A 李娇
%A 史鸿飞
%A 高欲燃
%J 微生物学报
%D 2009
%I 
%X Abstract: Objective]: In order to construct the recombinant bovine hepervirus-1(BHV-1) which expressed foot and mouth disease virus (FMDV)VP1 gene, we constructed a BHV-1 gE gene transfer vector by inserting the synthetic VP1 gene of FMDV (O/China/99) under the immediate-early promoter of cytomegalovirus. Methods]: The mixtures of parental virus (BHV-1/gE-/LacZ+ ) DNA and transfer vector was transfected into bovine turbinate cells using calcium phosphate-mediated transfection. Then the propagated viruses were harvested. The recombinant BHV-1 (designated BHV-1/gE-/VP1) was obtained by selection for white virus plaques. Results]: PCR results showed that VP1 gene was successfully inserted into the genome of BHV-1/gE-. The expression of VP1 in infected cells was proved by indirect immunofluorescence assay and Western blotting. Conclusion]: The research provided a basis for development of BHV-1 vector vaccines for FMD and other important bovine infectious diseases.
%K Keywords: FMDV
%K VP1 gene
%K Bovine hepervirus-1
%K Calcium phosphate-mediated transfection<br>关键词：
%K 口蹄疫病毒
%K VP1基因
%K 牛疱疹病毒1型
%K 磷酸钙介导转染
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=DF9A562FCD62DC6AD1F54D2062CD2A0B&yid=DE12191FBD62783C&vid=2A3781E88AB1776F&iid=94C357A881DFC066&sid=2E4E3741E8FB64E9&eid=6D25DD85174CF6DB&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=0