%0 Journal Article
%T Extraction of mitochondrial DNA of Tilletia indica Mitra and application of the ATP6 gene on fungal phylogenetic analysis<br>小麦印度腥黑粉菌线粒体DNA提取及其ATP6基因在真菌遗传发育分析中的应用
%A ZHOU Ye-qin
%A YI Jian-ping
%A ZHOU Guo-liang
%A DONG Ying
%A <br>周业琴
%A 易建平
%A 周国梁
%A 董英
%J 微生物学报
%D 2007
%I 
%X Tilletia indica Mitra,an important pest around the world which is the causative agent of Karnal Bunt of wheat,was very close to Tilletia walkeri phylogenetically and morphologically. 69.6ng mitochondrial DNA (mtDNA) of Tilletia indica obtained from 6.4mg total DNA by the method of CsCl/bisbenzimide density gradient ultracentrifugation can be used for electrophoresis,cloning,enzyme restriction analysis and PCR amplification. The ATP6 gene sequence was cloned from the fragment of mtDNA and sequenced for analysis of phylogenetic tree with the other related sequences in GenBank by the software PAUP to reveal the phylogenetic relationships within the Tilletia species.
%K Tilletia indica
%K mitochondrial DNA
%K phylogenetic analysis<br>小麦印度腥黑粉菌
%K 线粒体DNA
%K 系统发育分析
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=4247AA1C46917F73&yid=A732AF04DDA03BB3&vid=F4B561950EE1D31A&iid=94C357A881DFC066&sid=92DE343A8428AA81&eid=F7C11D7E3E8C5D3F&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=23