%0 Journal Article
%T Fusion expression of SLT-IIeB gene and FedF gene of Ee in Escherichia coli and its immunogenicity<br>大肠杆菌Ee株SLT-IIeB与FedF基因的融合表达及表达产物的免疫原性研究
%A LIU Guo-ping
%A WU Bin
%A LIN Yi-yuan
%A LIU Meng-yuan
%A CHEN Huan-chun
%A <br>刘国平
%A 吴斌
%A 林艺远
%A 刘梦元
%A 陈焕春
%J 微生物学报
%D 2007
%I 
%X The DNA fragment encoding the truncated SLT-IIeB and FedF of Ee strain were fused to the downstream of glutathione S-transferase (GST) of pGEX-KG expression vector, resulting in the fusion expression plasmid pKSF. After transformed into E. coli BL21 (DE3) and induced by IPTG, the results of SDS-PAGE showed that the GST-SF fusion protein was expressed in high level. Western blot was performed to confirm that the expressed fusion protein could specifically react with mouse anti-SLT-IIeB antiserum, mouse anti-FedF antiserum and moue anti-GST monoclonal antibody respectively. The fusion protein was further purified and used as an antigen for preparation of immune serum. The anti-sera of GST-SF were able to restrain the toxicity of SLT-IIe to Vero-E6 cells and inhibit the adhesin of F18 fimbriae to brush borders of swine in vitro. Groups of SPF KM mice were vaccinated subcutaneously at 0 week with 25 micrograms and at 2 weeks with 25 micrograms of purified GST-SF, GST-B, GST-F and challenged intraperitoneally with volume of 5 LD50 Ee strain. The results show the fusion protein GST-SF had more shrong immunogenicity and better protection against Ee strain.
%K SLT-IIeB
%K FedF<br>Ee株
%K 融合表达
%K 免疫原性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=ED2CC2FD33A6F13BEB84E967F30E4D15&yid=A732AF04DDA03BB3&vid=F4B561950EE1D31A&iid=B31275AF3241DB2D&sid=3F10405738B4D004&eid=AE9D5375B9B2D7F2&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=22