%0 Journal Article
%T Isolation and identification of surfactin producing Bacillus Subtilis strain and its effect of surfactin on crude oil<br>一株产生脂肽的枯草芽孢杆菌的分离鉴定及脂肽对原油的作用
%A Dawei Wang
%A Yongjian Liu
%A Zhongping Lin
%A Zhenyu Yang
%A Chunlei Hao
%A <br>王大威
%A 刘永建
%A 林忠平
%A 杨振宇
%A 郝春雷
%J 微生物学报
%D 2008
%I 
%X Strains producing biological surfactants were isolated from formation water of Daqing oil field, Heilongjiang Province, China. From which a lipopeptide producing strain ZW-3 was screened out by PCR of the sfp gene. The morphology, cultural characteristics, physiological, biochemical properties and chemotaxonomy of strain ZW-3 were studied. The strain is rod shaped (0.7-0.8 microm x 2-3 microm), gram-positive, spore-forming and aerobic bacteria. Its (G+C) content was determined to be 42.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequence demonstrated that it was closely related to the genus Bacillus subtilis, and the metabolites of strain ZW-3 was analyzed by thin layer chromatography and high performance liquid chromatography, the result indicated that the biosurfactant strain ZW-3 produced was surfactin. It could reduce surface tension of bacterial fermentation culture medium and water/oil- interfacial tension from 68.82 mN/m to 24.88 mN/m and from 23.53 mN/m to 4.57 mN/m, respectively, and its mixture with 1.8% NaOH could reduce water/oil- interfacial tension to an ultra low level (1.2 x 10(-3) mN/m), Its critical micelle concentration (CMC) was tested to be 33.3 mg/L (3.24 x 10(-5) mol/L)at 25 degrees C, and it had excellent emulsifying (2.89U) and foaming property. All these results showed that this biosurfactant had great potential in pharmaceutics, environmental protection, cosmetic, oil recovery and many other application fields.
%K Bacillus subtilis
%K polyphasic taxonomy
%K lipopeptide
%K biosurfactant
%K physico-chemical properties
%K crude oil<br>枯草芽孢杆菌
%K 多相分类
%K 脂肽
%K 生物表面活性剂
%K 理化性质
%K 原油
%K 脂肽
%K 枯草芽孢杆菌
%K 分离鉴定
%K 原油
%K 作用
%K crude
%K oil
%K effect
%K strain
%K Bacillus
%K Subtilis
%K producing
%K identification
%K 前景
%K 应用
%K 提高石油采收率
%K 发泡性能
%K 乳化活性
%K 临界胶束浓度
%K 油水界面张力
%K 溶液复配
%K NaOH
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=01ABF4EE63493D50148372FFA477F019&yid=67289AFF6305E306&vid=B6DA1AC076E37400&iid=38B194292C032A66&sid=8ED630AD8C61FAE8&eid=A2745AA1110798CA&journal_id=0001-6209&journal_name=微生物学报&referenced_num=1&reference_num=15