%0 Journal Article
%T Screening of a Lactobacillus casei with monoamine oxidase-inhibitory activity<br>一株具有抑制单胺氧化酶作用的干酪乳杆菌筛选
%A Hao Wang
%A Benheng guo
%A Zhengjun Wu
%A Yin-yu Wang
%A <br>王豪
%A 郭本恒
%A 吴正钧
%A 王荫榆
%J 微生物学报
%D 2010
%I 
%X Abstract: Objective] To screen for monoamine oxidase (MAO)-inhibiting lactobacillus from healthy human dejecta in an in vitro model, and to provide reference for anti-ageing study in vivo of lactobacillus in the future. Methods] The monoamine oxidase inhibitory model in vitro was applied to screen fermented supernatant and cell-free extracts originated from lactic acid bacteria, and two indices based on screened samples were determined, including biological dosage-effect and the effect of pre-incubated time between MAO and inhibitor on suppression rate. Meanwhile, with membrane separation technology, MAO inhibition from different molecular weight range of samples was measured. Screened strain JH-23 was investigated as target probiotic lactobacillus, identified through the sequence analysis of 16S rDNA gene and API system. Results] The MAO inhibitory rate of cell-free extracts produced by strain JH-23 reached 33.7 %. After samples being vacuum freeze-dried, MAO inhibitory rate was up to 53.2 % when the reactive concentration was 16 mg/mL. The inhibition was significantly enhanced with the duration of pre-incubation increased, and then the inhibitory effect became steady after 30 minutes. Crude samples were dialyzed for 48 hours, MAO inhibitory effect of dialysates was significantly increased compared with pre-dialysis. The result of bacteria identification demonstrated that strain JH-23 was ascribed to Lactobacillus casei. Conclusion] A new screening model in vitro, regarding monoamine oxidase as a target enzyme, was explored in this study. This model was characterized by convenience, rapidity and high sensitivity, and it could be useful for the following anti-aging research in vivo. The cell-free extracts of Lactobacillus casei JH-23 was inhibitory to MAO, and the intracellular small-molecules played a major role in the inhibition.
%K Keywords: Lactobacillus
%K monoamine oxidase
%K screening model in vitro<br>关键词：乳酸菌
%K 单胺氧化酶
%K 体外筛选模型
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=B0677693150649BF4ACFE2E07D40B8A0&yid=140ECF96957D60B2&vid=771152D1ADC1C0EB&iid=0B39A22176CE99FB&sid=2BA123C6EB9D54C2&eid=38685BC770C663F2&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=0