%0 Journal Article
%T In vivo replacement of phosphatidylethanolamine by phosphatidylcholine affects biological function of E. coli<br>活体内磷脂酰胆碱替代磷脂酰乙醇胺影响E. coli细胞的功能
%A Bin Wu
%A Qing Ye
%A Yang Li
%A Changyu Li
%A Xuejing Yu
%A Shu Zhou
%A Xingguo Wang
%A <br>吴彬
%A 叶青
%A 李洋
%A 李昌瑜
%A 喻雪婧
%A 周舒
%A 王行国
%J 微生物学报
%D 2010
%I 
%X Abstract: Objective] We investigated differences in morphology, physiology, periplasmic proteins and phagocytosis by macrophage among E. coli strains AD93(PE-PC-), AD93/ptac67(PE-PC+), Top10/ptac66(PE+PC+) and the wild types in order to understand if phosphatidylcholine (PC) can substitute phosphatidylethanolamine (PE) in vivo. Methods] Bacterial cells were observed under microscope after staining with Gram-staining kit or by electron microscope. Bacterial growth under different conditions was monitored by measuring the absorbance at the wavelength of 600 nm. Periplasmic proteins were analyzed using SDS-PAGE and 2-D electrophoresis. Bacterial adherence and phagocytosis by macrophage were also examined by using murine RAW264.7 macrophage. Results] 100% bacterial cells in AD93/pDD72 were bar-shaped but 25% AD93/ptac67 cells came out as long filaments. Different from AD93/pDD72, AD93/ptac67 and AD93 required Mg2+or Ca2+ for growth. Moreover, AD93/ptac67 displayed a different pattern of periplasmic proteins on a 2-D gel and a low relatively phagocytic efficiency in the phagocytosis test when compared to AD93/pDD72 and AD93. Both Top10/ptac66 and the wild-type Top10/ptac85 cells were bar-shaped under microscope, but the former showed noticeably difference in the outer-layer structure of cell wall, and its stress resistance and periplasmic protein composition were also different from those of the latter. Conclusion] Substitution of phosphatidylethanolamine with phosphatidylcholine in E. coli cells is unable to restore the phonotype of PE- mutant to the wild type. Biological functions of PE and PC are different, and phosphatidylcholine cannot substitute phosphatidylethanolamine in vivo.
%K 关键词：磷脂酰胆碱
%K 磷脂酰乙醇胺
%K 大肠杆菌
%K 膜磷脂替换
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=817E68EF24EAC808FC682DF2FDBADF2B&yid=140ECF96957D60B2&vid=771152D1ADC1C0EB&iid=708DD6B15D2464E8&sid=60784034DD03F1E1&eid=BD9BFF5428C0CED6&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=0