%0 Journal Article
%T College of Food & Biotechnology Engineering, Zhejiang Gongshang University, Hangzhou 310035, China
植物乳杆菌ZJ316生产细菌素
%A Jingliang Li
%A Dafeng Song
%A Qing Gu
%A
李景良
%A 宋达峰
%A 顾青
%J 微生物学报
%D 2008
%I
%X OBJECTIVE: To enhance the production of plantaricin by Lactobacillus plantarum ZJ316 isolated from infant feces. METHODS: We analyzed fermentation parameters influencing cell growth and plantaricin production with different medium composition and under different cultivation conditions. RESULTS: MRS (DE MAN, ROGOSA, SHARPE) medium was more suitable for producing bacteriocin than other media. The maximum plantaricin production was obtained in modified MRS medium containing 10 g/L maltose and 10 g/L glucose, 10 g/L yeast extract, 10 g/L tryptone and 2g/L tri-ammonium citrate, 1 mL/L Tween80, 6 g/L K2HPO4.3H2O, 5 g/L sodium acetate, 0.2 g/L magnesium sulfate and 0.05 g/L manganese sulfate. The optimal initial pH and temperature for plantaricin production were 6.5 and 30 degrees C for 24 h. CONCLUSION: After optimizing, the production of plantaricin was increased 2.3-fold using the optimized medium, compared with in the basic MRS medium.
%K plantaricin
%K cultivation conditions
%K optimization
细菌素
%K plantaricin
%K ZJ316
%K 培养条件
%K 植物乳杆菌
%K 规模化生产
%K 细菌素
%K Lactobacillus
%K plantarum
%K production
%K 产量提高
%K 静置培养
%K 硫酸锰
%K 硫酸镁
%K 乙酸钠
%K 柠檬酸三铵
%K 蛋白胨
%K 酵母提取物
%K 麦芽糖
%K 葡萄糖
%K 配方
%K 优化
%K 培养基
%K 结果
%K 影响
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=D9A803AA538B2DA12D4A8E184F90A961&yid=67289AFF6305E306&vid=B6DA1AC076E37400&iid=B31275AF3241DB2D&sid=F2947E14627CD734&eid=CE504F5B1E192581&journal_id=0001-6209&journal_name=微生物学报&referenced_num=2&reference_num=15