%0 Journal Article %T College of Food & Biotechnology Engineering, Zhejiang Gongshang University, Hangzhou 310035, China
植物乳杆菌ZJ316生产细菌素 %A Jingliang Li %A Dafeng Song %A Qing Gu %A
李景良 %A 宋达峰 %A 顾青 %J 微生物学报 %D 2008 %I %X OBJECTIVE: To enhance the production of plantaricin by Lactobacillus plantarum ZJ316 isolated from infant feces. METHODS: We analyzed fermentation parameters influencing cell growth and plantaricin production with different medium composition and under different cultivation conditions. RESULTS: MRS (DE MAN, ROGOSA, SHARPE) medium was more suitable for producing bacteriocin than other media. The maximum plantaricin production was obtained in modified MRS medium containing 10 g/L maltose and 10 g/L glucose, 10 g/L yeast extract, 10 g/L tryptone and 2g/L tri-ammonium citrate, 1 mL/L Tween80, 6 g/L K2HPO4.3H2O, 5 g/L sodium acetate, 0.2 g/L magnesium sulfate and 0.05 g/L manganese sulfate. The optimal initial pH and temperature for plantaricin production were 6.5 and 30 degrees C for 24 h. CONCLUSION: After optimizing, the production of plantaricin was increased 2.3-fold using the optimized medium, compared with in the basic MRS medium. %K plantaricin %K cultivation conditions %K optimization
细菌素 %K plantaricin %K ZJ316 %K 培养条件 %K 植物乳杆菌 %K 规模化生产 %K 细菌素 %K Lactobacillus %K plantarum %K production %K 产量提高 %K 静置培养 %K 硫酸锰 %K 硫酸镁 %K 乙酸钠 %K 柠檬酸三铵 %K 蛋白胨 %K 酵母提取物 %K 麦芽糖 %K 葡萄糖 %K 配方 %K 优化 %K 培养基 %K 结果 %K 影响 %U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=D9A803AA538B2DA12D4A8E184F90A961&yid=67289AFF6305E306&vid=B6DA1AC076E37400&iid=B31275AF3241DB2D&sid=F2947E14627CD734&eid=CE504F5B1E192581&journal_id=0001-6209&journal_name=微生物学报&referenced_num=2&reference_num=15