%0 Journal Article
%T Expression of a modified antimicrobial peptide BhSGAMP-1-S (Bradysia hygida) in Escherichia coli and characterization of its activity<br>迟眼蕈蚊抗菌肽BhSGAMP-1-S在大肠杆菌中的优化表达及其活性分析
%A Jue Wang
%A Qiurong Gao
%A Qiang Liu
%A Linping Xiang
%A Yuqin Wang
%A Dun Wang
%A <br>王珏
%A 高秋荣
%A 刘强
%A 项林平
%A 王玉芹
%A 王敦
%J 微生物学报
%D 2010
%I 
%X bstract: Objective] Bradysia hygida salivary glands antimicrobial peptide 1 (BhSGAMP-1) is one of the antimicrobial peptides involved in a preventive mechanism of defense of the fly Bradysia hygida. To know better about the molecular characterization of this antimicrobial peptide, we expressed and purified the modified BhSGAMP-1-S and investigated its antimicrobial activity. Methods] We synthesized the gene of BhSGAMP-1-S designed with preferred codons of Escherichia coli and expressed it as a fusion protein in E.coli TB1 by using pMAL-c2X as vector. We purified the fusion protein using amylase resin affinity chromatography. In addition, we cleaved the fusion protein by enterokinase and the released recombinant BhSGAMP-1-S was separated by size exclusion chromatography, then followed by reversed-phase high performance liquid chromatography. We analyzed the antimicrobial activities of the purified recombinant BhSGAMP-1-S by bioassay. Results] The fusion protein was mostly expressed in soluble form under the optimized conditions. The recombinant BhSGAMP-1-S was produced with a pure yield of 0.38 mg/100 mL culture medium. Antimicrobial assays demonstrated that the recombinant BhSGAMP-1-S was active against several Gram-positive and Gram-negative bacteria and fungi. Conclusion] It appears to be first successful production of the recombinant BhSGAMP-1-S from fly Bradysia hygida. Data presented here confirm that the recombinant BhSGAMP-1-S is now ready for further studying and characterize their antimicrobial properties.
%K Keywords: antimicrobial assays
%K antimicrobial peptides
%K BhSGAMP-1-S
%K Bradysia hygida
%K purification
%K soluble expression<br>关键词：抑菌分析
%K 抗菌肽
%K BhSGAMP-1-S
%K 迟眼蕈蚊
%K 纯化
%K 可溶性表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=4557BE1398936C7DBCE74A1606392119&yid=140ECF96957D60B2&vid=771152D1ADC1C0EB&iid=9CF7A0430CBB2DFD&sid=38FD6970749494EF&eid=8339CA1DD1F3C6AB&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=0