%0 Journal Article
%T Phage Resistance of Corynebacterium crenatum Conferred by the Restriction and Modification System cglI<br>限制修饰系统cglI赋予钝齿棒杆菌抗噬菌体功能活性
%A Yongfei Hu
%A Tiemin Li
%A Zhiyong Yang
%A Bo Zhang
%A Yu Li
%A <br>胡永飞
%A 李铁民
%A 杨智勇
%A 张博
%A 李玉
%J 微生物学报
%D 2008
%I 
%X In order to prevent phage contamination in amino acid fermentation, we introduced the restriction and modification system cglI gene complex into Corynebacterium crenatum and studied their phage-resistance. The cglI gene complex was amplified from Corynebacterium glutamicum by PCR and constructed into pJL23 vector. The recombinant strains were obtained by transformation of the recombinant plasmid pJL23-cglI into C. crenatum. Results showed that the recombinant strains possessed strong phage- resistance activity and broad phage-resistance spectrum, demonstrating the feasibility of using cglI gene complex for construction of phage-resistance recombinant C. crenatum strains and presenting a powerful way to solve the problem of phage contamination in amino acid fermentation industry.
%K restriction and modification system
%K cglI gene complex
%K Corynebacterium crenatum
%K bacteriophage
%K genetic engineered strain<br>限制修饰系统
%K cglI基因复合体
%K 钝齿棒杆菌
%K 噬菌体
%K 基因工程菌
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=940BF22C2BED968E244E3AA0E1D80D12&yid=67289AFF6305E306&vid=B91E8C6D6FE990DB&iid=94C357A881DFC066&sid=8D71AF42ACD39979&eid=849F08713EB6820A&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=0