%0 Journal Article
%T Differential expression protein between the thuringiensin-yield Bacillus thur-ingiensis strain CT-43 and its mutants<br>苏云金芽胞杆菌产苏云金素菌株CT-43及其 缺失突变株的差异蛋白
%A Chengliang Guo
%A Zhenfei Hu
%A Xiaoyan Liu
%A Ziquan Yu
%A Lifang Ruan
%A Ming Sun
%A Ziniu Yu
%A <br>郭承亮
%A 胡振飞
%A 刘晓艳
%A 余子全
%A 阮丽芳
%A 孙明
%A 喻子牛
%J 微生物学报
%D 2008
%I 
%X Objective] The purpose of this research is trying to uncover the biosynthetic or metabolic relative protein of thuringiensis by proteomics. Methods] We conducted differential expression analysis between the high thuringiensin-yield Bacillus thuringiensis strain CT-43 and its mutants, high production strain CT-43-1C and non-production strain BMB0806, based on the 2-D gel. Then through mass spectrum (MS) identification and bio-informatics we analyzed the detected proteins. Results] Thirteen spots were selected to be detected by the MS, and nine of them were identified. Among them, six proteins including in the basal metabolism pathway were possibly deduced that relative with the synthesis or metabolism of thuringiensin production. Conclusion] There were six proteins found to be connected with the influential role protein of biosynthesis and metabolic of thuringiensin production by comparative proteomics. This research provides the evidence of thuringiensis gene cluster cloning and biosynthetic analysis.
%K Bacillus thuringiensis
%K Thuringiensin
%K Proteomics
%K Differential protein<br>苏云金芽胞杆菌
%K 苏云金素
%K 蛋白质组学
%K 差异蛋白
%K 苏云金芽胞杆菌
%K 高产菌株
%K 缺失突变株
%K 差异蛋白
%K mutants
%K strain
%K Bacillus
%K thuringiensis
%K protein
%K expression
%K 证据
%K 验证
%K 合成途径
%K 克隆
%K 基因簇
%K 显示
%K 生物信息学分析
%K 差异显著
%K 发现
%K 野生型
%K 结果
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=A0C32F2AA0E6D5E235AB470EBD2E0754&yid=67289AFF6305E306&vid=B6DA1AC076E37400&iid=DF92D298D3FF1E6E&sid=714E16F7CF56F343&eid=3A9B6DFDAB34D091&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=18