%0 Journal Article
%T Construction of a Pseudomonas aeruginosa random promoter library and identification of novel iron-regulated genes by high-throughput library screening<br>铜绿假单胞菌随机启动子库的构建及受铁调控基因的筛选和鉴定
%A Yan Wang
%A Jinhua Wei
%A Lixin Shen
%A Kangmin Duan
%A <br>王琰
%A 魏金花
%A 沈立新
%A 段康民
%J 微生物学报
%D 2008
%I 
%X OBJECTIVE: Iron is an essential element for bacterial survival and pathogenesis. Using a random promoter library we screened for iron-responsive genes in P. aeruginosa genome. METHOD: Plasmid pMS402 containing a promoterless luminescence reporter, LuxCDABE, was used as the vector to construct a P. aeruginosa random promoter library with a size of about 5700 clones. It enabled real-time, in vivo high-throughput gene expression profiling at genomic scale. RESULTS: Two previously reported iron-regulated genes were identified, and ten new iron-regulated genes were uncovered. The genes encoding dihydrolipoamide acetyltransferase, phosphogluconate dehydratase and Fe (III) dicitrate transporter were found to be iron-regulated together with four function-unknown genes and three putative protein encoding genes. CONCLUSION: These results provide a basis for elucidating the complex iron regulation network in P. aeruginosa and help our understanding of the roles of iron in bacterial physiology and pathogenesis. The random promoter library system also offers a useful tool in bacterial genomic studies.
%K Pseudomonas aeruginosa
%K random promoter library
%K iron
%K gene regulation<br>铜绿假单胞菌
%K 随机启动子库
%K 铁
%K 基因调控
%K 铜绿假单胞菌
%K 随机
%K 启动
%K 调控基因
%K 筛选
%K screening
%K library
%K genes
%K novel
%K identification
%K promoter
%K random
%K Pseudomonas
%K aeruginosa
%K 基因表达
%K 细菌基因组
%K 理论
%K 机制
%K 调控作用
%K 致病性
%K 生理
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=6ACC272F1EEA32F0F9CF16305FA59C7C&yid=67289AFF6305E306&vid=B6DA1AC076E37400&iid=DF92D298D3FF1E6E&sid=AD0A5DE51C29AB9F&eid=B84F2E0A99FDC89A&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=20