%0 Journal Article
%T Co-expressed GP5 and M proteins of porcine reproductive and respiratory syndrome virus can form heterodimers<br>体外共表达的猪繁殖与呼吸综合征病毒GP5和M蛋白可形成异源二聚体
%A MA Yan
%A JIANG Yun-bo
%A XIAO Shao-bo
%A FANG Liu-rong
%A CHEN Huan-chun
%A <br>马艳
%A 江云波
%A 肖少波
%A 方六荣
%A 陈焕春
%J 微生物学报
%D 2006
%I 
%X In order to investigate the characterization of in vitro co-expressed GP5 and M proteins of porcine reproductive and respiratory syndrome virus(PRRSV),eukaryotic expression plasmids pCI-ORF5(expressing GP5 protein alone),pCI-ORF6(expressing M protein alone),and pCI-ORF5/ORF6(co-expressing GP5 and M proteins) were constructed.After transient transfection,Western blot analysis under nonreducing condition demonstrated that co-expressed GP5 and M proteins could form disulfide-linked heterodimers(GP5-M) in transiently transfected BHK-21 cells.To further study the influence of GP5-M heterodimers formation on the subcellular localizations of GP5 or M proteins,green fluorescence protein(EGFP) and red fluorescence protein(RFP) were used as markers.The results of fluorescence distribution showed that co-expressed GP5-EGFP chimera and M-RFP chimera boosted the transport of GP5 from the endoplasmic reticulum(ER) to the Golgi complex,indicating that the formation of GP5-M heterodimers may be involved in posttranslational modification,transport,and subcellular localization of GP5.These results presented here lay foundation to further study the molecular mechanism of GP5-M heterodimer formation and its role in protective immunity of PRRSV.
%K PRRSV
%K ORF5
%K ORF6
%K Co-expression
%K Heterodimer<br>PRRSV
%K ORF5
%K ORF6
%K 双基因共表达
%K 异源二聚体
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=43851A347BBB0025&yid=37904DC365DD7266&vid=D997634CFE9B6321&iid=E158A972A605785F&sid=46C2A519EDDA03DD&eid=ADF3752CBFE933EB4E87F990C792EC05&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=12