%0 Journal Article
%T Expression of alkali-tolerant xylanase in Bacillus megaterium<br>耐碱性木聚糖酶基因在巨大芽孢杆菌中的表达及其酶学性质
%A Yihong Bao
%A Weifeng Liu
%A Yongzhi He
%A Zhiyang Dong
%A <br>包怡红
%A 刘伟丰
%A 何永志
%A 董志杨
%J 微生物学报
%D 2009
%I 
%X Abstract: Objective] We cloned xylanase-encoding gene xynA and its promoter from Bacillus pumilus, expressed in Bacillus megaterium and characterized the recombinant xylanase. Methods] We inserted the xylanase-encoding gene xynA and its promoter in Bacillus expression vector pWH1520 and pWG03 which was modified from pWH1520. We transformed the recombinant plasmid pWTEJX and pWGXYN into Bacillus megaterium, and obtained the recombinant stains BMJXH9 and BMGpp12. Enzymes produced by recombinant strains expressing xynA were produced in the medium. The xylanase activity produced by recombinant BMGpp12 was three times higher than that of BMJXH9. The recombinant xylanase had the original enzyme alkali-tolerant properties. Conclusion] Alkali-tolerant xylanase gene was successfully expressed and this provided a basis for further study of xylanase applied.
%K Keywords: Xylanase
%K Bacillus megaterium
%K Bacillus expression system<br>关键词：木聚糖酶
%K 巨大芽孢杆菌
%K 芽孢杆菌表达系统
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=9AA75C5704B04460AA4B6413426BDA7E&yid=DE12191FBD62783C&vid=2A3781E88AB1776F&iid=F3090AE9B60B7ED1&sid=FBB416F02256D8AA&eid=D98C4F25072149E5&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=0