%0 Journal Article
%T Detection and diversity analysis of rumen methanogens in the co-cultures with anaerobic fungi<br>共存于厌氧真菌分离培养液中瘤胃甲烷菌的检测及其多样性分析
%A CHENG Yan-fen
%A MAO Sheng-yong
%A PEI Cai-xia
%A LIU Jian-xin
%A ZHU Wei-yun
%A <br>成艳芬
%A 毛胜勇
%A 裴彩霞
%A 刘建新
%A 朱伟云
%J 微生物学报
%D 2006
%I 
%X Rumen methanogen diversity in the co-cultures with anaerobic fungi from goat rumen was analyzed. Mix-cultures of anaerobic fungi and methanogens were obtained from goat rumen using anaerobic fungal medium and the addition of penicillin and streptomycin and then subcultured 62 times by transferring cultures every 3 - 4d. Total DNA from the original rumen fluid and subcultured fungal cultures was used for PCR/DGGE and RFLP analysis. 16S rDNA of clones corresponding to representative OTUs were sequenced. Results showed that the diversity index (Shannon index) of the methanogens generated from DGGE profiles reduced from 1.32 to 0.99 from rumen fluid to fungal culture after 45 subculturing, with the lowest similarity of DGGE profiles at 34.7%. The Shannon index increased from 0.99 to 1.15 from the fungal culture after 45 subculturing to that after 62 subculturing, with the lowest similarity at 89.2% . A total of 5 OTUs were obtained from 69. clones using RFLP analysis and six clones representing the 5 OTUs respectively were sequenced. Of the 5 OTUs, three had their cloned 16S rDNA sequences most closely related to uncultured archaeal symbiont PA202 with the same similarity of 95 %, but had not closely related to any identified culturable methanogen. The rest two OTUs had their cloned 16S rDNA sequences sharing the same closest relative, uncultured rumen methanogen 956, with the same similarity of 97% .Their 16S rDNA sequences of these two OTUs also showed 97% similar to the closest identified culturable methanogen Methanobrevibacter sp. NT7. In conclusion, diverse yet unidentified rumen methanogen species exist in the co-cultures with anaerobic fungi isolated from the goat rumen.
%K Anaerobic fungi
%K Methanogens
%K DGGE
%K RFLP<br>厌氧真菌
%K 甲烷菌
%K DGGE
%K RFLP
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=CF6DF0CE1330A325&yid=37904DC365DD7266&vid=D997634CFE9B6321&iid=B31275AF3241DB2D&sid=D698D0190A84C2BD&eid=899CC9158FC43EF4&journal_id=0001-6209&journal_name=微生物学报&referenced_num=8&reference_num=15