%0 Journal Article
%T Identification of Legionella isolates from environmental water by using PCR combined with enzymatic digestion analysis<br>聚合酶链反应-酶切分型鉴定广州地区环境水源军团菌
%A Liwei Zhao
%A Chaohui Hu
%A Ruihong Xuan
%A Yangmin Liu
%A Qingyi Zhu
%A Juan Wang
%A Xiaoyong Zhan
%A <br>赵利伟
%A 胡朝晖
%A 宣瑞红
%A 刘洋敏
%A 朱庆义
%A 王娟
%A 詹晓勇
%J 微生物学报
%D 2010
%I 
%X Abstract: Objective] To evaluate polymerase chain reaction (PCR) combined with enzymatic digestion for identification of Legionella, and distribution of Legionella in environmental water systems in Guangzhou. Methods] Forty-four water samples collected in Guangzhou were cultivated for Legionella were identified by PCR-enzymatic digestion, 16S rDNA and mip gene sequencing analysis. Results] Sixty-six strains of Legionella pneumophila and 46 Non-L. pneumophila were identified by PCR-enzymatic digestion and sequencing analysis. Forty-six strains of Non-L. pneumophila included 20 strains of L. feeilei, 17 L. gormanii, 7 L. oakridgensis and 2 L. longbeachae. Conclusion] PCR combined with enzymatic digestion is a simple, rapid, and specific method for the identification of Legionella. L. pneumophila was distributed widely, followed by L. feeilei, L. gormanii, L. oakridgensis and L. longbeachae, in environmental water in Guangzhou area.
%K Keywords: Legionella
%K Environmental water
%K PCR combined with enzymatic digestion
%K Gene sequencing<br>关键词：军团菌
%K 环境水源
%K 聚合酶链反应-酶切分型
%K 基因测序
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=79001B7E6D50C2665CC72F6EA8AD26C3&yid=140ECF96957D60B2&vid=771152D1ADC1C0EB&iid=708DD6B15D2464E8&sid=B9B1149CB26916EC&eid=69E0A22DA7775B50&journal_id=0001-6209&journal_name=微生物学报&referenced_num=1&reference_num=0