%0 Journal Article
%T Construction of deinococcal bacteria - Escherchia coli shuttle vector and expression of Luciferase gene<br>抗辐射菌属-大肠杆菌间的穿梭载体的构建及荧光基因的表达
%A Zhenli Tu
%A Rujie Zhong
%A Jiagang Wang
%A <br>屠振力
%A 钟儒杰
%A 王家刚
%J 微生物学报
%D 2009
%I 
%X Abstract: 0bjective] To express Luciferase gene in Escherchia coli through developed Deinococcal bacteria - E. coli shuttle expression vector. Methods]The D. bacteria - E. coli shuttle expression vector pZT17 was constructed based on plasmids of pUE30, pGBM5 and pKatCAT. Then pZT17 with lux+ from Photinus pyralis was used to transform into D. grandis and E.coli. The recombinant strains were induced separately. Results] Based on a small cryptic plasmid from Deinococcus radiopugnans, a shuttle vector between Escherichia coli and deinococcal bacteria was constructed. The plasmid vector could stably aintained in Deinococcus grandis under non-selective conditions. Moreover, it is showed that a luciferase gene was highly expressed both observed in D. grandis and E.coli. Conclusions]The D. bacteria - E. coli shuttle vector was constructed successfully, the developed shuttle vector makes it possible to induce expression of DNA damage and repair gene from Deinococcus species.
%K Keyword: Deinococcal bacteria
%K Escherichia coli
%K shuttle vector
%K lux+
%K expression vector<br>关键词：抗辐射菌
%K 大肠杆菌
%K 穿梭载体
%K 荧光素酶基因
%K 表达载体
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=BFD1273C19353DB603C8AA860DEAAF40&yid=DE12191FBD62783C&vid=2A3781E88AB1776F&iid=94C357A881DFC066&sid=E513158F1BE1471F&eid=C7461453A367FC85&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=14