%0 Journal Article %T Identification and Quantitation of Ceramides in Yeast Yarrowia lipolitica
解脂假丝酵母中神经酰胺的鉴别和定量分析 %A ZHOU Quan %A CHEN Guo-Qiang %A
周全 %A 陈国强 %J 微生物学报 %D 2004 %I %X Lipids in yeast Yarrowia lipolitica was separated by preparative Thin Layer Chromatography (TLC) to obtain ceramide. Mass Spectrum (MS) and tandem-MS were applied to confirm the existence of the ceramide in Y. lipolitica and to reveal its structure. Combined with High Performance Liquid Chromatography and Evaporative Light Scattering Detector (HPLC/ELSD), for the first time, the ceramide content with 24 carbons in Fatty Acid (FA) side chain was determined in Y. lipolitica. %K Yarrowia lipolitica %K Ceramide %K HPLC %K ELSD tandem-MS
解脂假丝酵母 %K 神经酰胺 %K 高效液相色谱 %K 二级质谱 %K 蒸发光散射检测器 %U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=2DBFDEFC181A988D&yid=D0E58B75BFD8E51C&vid=1AE5323881A5ECDC&iid=94C357A881DFC066&sid=15F713CDE16A589C&eid=D02611D1F8166C9A&journal_id=0001-6209&journal_name=微生物学报&referenced_num=2&reference_num=14